conclusions in this study, the optimized truncated orf2 protein was expressed in e. coli successfully and the highly purified protein was obtained, which can be a potential vaccine candidate and as an antigen in elisa to diagnose hev infections. results the subcloning was confirmed by pcr, restriction enzyme digestion, and dna sequencing of recombinant plasmid pet30a-orf2.2. the results obtaine...

background the development of an effective subunit vaccine against brucellosis is a research area of intense. but optimization of recombinant proteins production in escherichia coli and content of endotoxins associated with final recombinant proteins are very important. objectives in the present study, expression and purification of brucella melitensis rhsp and rtf were optimized to reduce endo...

the rhizome of anemarrhena asphodeloides is used as food and traditional chinese medicine for its hypoglycemic effect. the aim of this study was to investigate the isolation, purification and hypoglycemic activity of anemaran as the active component. the influence factors (isolation duration, ratio of residuals to water and extracting times) during the isolation process were evaluated. the opti...

abstract aims: one of the consequences of taking ground water into surface is changing its chemical quality, specially increasing the concentration of dissolved salts. this research was performed in order to analyze growth possibility of heterotrophic bacteria in the membrane of active desalination plants in kashan city, iran. instrument & methods: this descriptive cross-sectional study was don...

buthotus schach finally, according to our results venom as well as fractions f4 and f6 act mostly prejunctionally on ach release. more attempt is carrying out to study their effects on ion channel activities. is one of the most dangerous scorpions in tropical part of iran. the effects of its crude venom at 1, 3, 10 µg/ml and its obtained fractions by gel filtrations were investigated on neuromu...

background: toxoplasmosis is a worldwide-distributed infection which is mostly asymptomatic but can cause serious health problems in congenitally-infected newborns and immunecompromised individuals. research is undergoing both to improve toxoplasma serological tests, which play the main role in laboratory diagnosis of the infection, and develop an effective vaccine to prevent the infection. som...

the photocatalytic decomposition of trichloroethylene (tce) and tetrachloroethylene (pce)in water was studied. tce and pce were present in water at very low concentrations of about 15 μg/dm3. thisconcentration level corresponds to the one occasionally occurring in groundwater. results of this researchconfirmed usefulness of the photocatalytic process for successful removal of low-concentrated t...

introduction: iranian scorpion species are classified in buthidae and scorpionidae with 16 genera and 25 species. in iran, similar to other parts of the world, there are a few known species of scorpions responsible for severe envenoming. mesobuthus eupeus is the most common species in iran. its venom contains several toxin fractions which can affect the ion channel. in this study purification, ...

in this study escherichia coli de3 containing expression vector (pet21a) with cloned persian sturgeon growth hormone (psgh) gene was grown in 10 ml lb broth on a 150 rpm shaker, at the temperature of 37 °c. at the late log phase (determined by od standard curve) 100 μl isopropyl β-d-1-thiogalactopyranoside (iptg) was added for induction of gh synthesis. samples were taken every 2 hours and afte...