A laboratory study of the viability of selected microorganisms in a hydrocarbon fuel medium was carried out on 19 species of microorganisms, representative of the types found as natural contaminants in aircraft fuels. More species remained viable when inoculated in pure cultures than when inoculated in mixed (composite) cultures. Of the 19 species selected, 10 were still viable after 3 months a...

a field experiment was conducted at research station of zabol university in 2012. the experiment carried out as split plot on basis of a randomized complete block design with three replications. the main and sub factors included tillage systems (no, reduced, and conventional tillage) and planting ratios of intercropping (pure culture of roselle, pure culture of green gram, 25% roselle + 75% gre...

Antibiotic activities of culture filtrates from twenty filamentous fungi, isolated from wood submerged in tropical freshwater habitats, were tested against a pathogenic strain of Candida albicans. These taxa were fermented separately in liquid medium in pure culture, while another set was fermented with the addition of a fluconazole-sensitive strain of Candida albicans in mix-culture. Fermentat...

OBJECTIVES in vitro studies commonly use single bacterial isolates for testing antibiotic susceptibilities. However, interspecies effects that may arise when mixed infections are treated with antibiotics can obviously not be investigated by this approach. In the study presented here, the effect of ceftazidime against a model microbial community consisting of Pseudomonas aeruginosa, Burkholderia...

The anaerobic bacterium Syntrophus aciditrophicus metabolized benzoate in pure culture in the absence of hydrogen-utilizing partners or terminal electron acceptors. The pure culture of S. aciditrophicus produced approximately 0.5 mol of cyclohexane carboxylate and 1.5 mol of acetate per mol of benzoate, while a coculture of S. aciditrophicus with the hydrogen-using methanogen Methanospirillum h...

Methods are detailed for isolating highly pure populations of spermatogonial stem cells from primary cultures of testis cells prepared from 22- to 24-day-old rats. The procedure is based on the principle that testicular somatic cells bind tightly to plastic and collagen matrices when cultured in serum-containing medium, whereas spermatogonia and spermatocytes do not bind to plastic or collagen ...