To examine the cross-talk between NK cells and DCs in hepatitis C virus (HCV) infection, we isolated monocytes from 20 chronic HCV patients healthy controls. Monocytes were used to generate immature which pulsed with peptides (core, NS3–NS4, NS5). Four different cocultures carried out: E1, both a patient; E2, control cocultured E3, patient control; E4, control. Using flow cytometry, assessed ef...

Abstract The etching characteristics were studied via time-modulation bias (bias pulsing) by varying the pulsing parameters. etch profiles verified using polysilicon gate structures with dense and isolated patterns. Ion energy was defined as peak-to-peak voltage ( V pp ) controlled RF power. durations of on period off (off time) adjusted pulse frequency duty cycle. Profile evolution observed in...

Floriculture is among Kenya’s top foreign exchange earners. Gladiolus is one of the four famous cut flowers in the world whose popularity in Kenya is attributed to its heat tolerance, many spike forms and color combinations. However, the perishable nature of the flower renders it vulnerable to huge post-harvest losses. The study aimed to evaluate pulsing effect of cut Gladiolus</em...

Where B is the ratio variance to average, p is the after pulsing probability, I is the intensity in photon counts/s and t is the dead time. Ignoring for the moment the after pulsing effect, in the absence of other effects, the value of B will decrease linearly with the intensity with a slope given by 2t. The following graph is an example for the expected changes in B as a function of intensity ...

Non-normality can underlie pulse dynamics in many engineering contexts. However, its role in pulses generated in biomolecular contexts is generally unclear. Here, we address this issue using the mathematical tools of linear algebra and systems theory on simple computational models of biomolecular circuits. We find that nonnormality is present in standard models of feedforward loops. We used a g...

We show that self-induced oscillations at frequencies above GHz and with a high spectral purity can be obtained in a silicon photonic crystal nanocavity under optical pumping. This self-pulsing results from the interplay between the nonlinear response of the cavity and the photon cavity lifetime. We provide a model to analyze the mechanisms governing the onset of self-pulsing, the amplitudes of...

Pixelated Photon Detectors (PPDs) are the most promising semiconductor photodetectors in recent years. One of the issues with the PPD is its high noise rate. As well as random noise, PPD also exhibits so called after-pulsing and optical crosstalk, and these limit the applicable range of its gain as well as its size. By accurately measuring each of these causes of noises independently, we quanti...