Statistical experimental designs were applied to optimize the fermentation medium for exopolysaccharide (EPS) production. Plackett-Burman design was applied to identify the significance of seven medium variables, in which sweet potato and yeast extract were found to be the significant variables for EPS production. Central composite design was applied to evaluate the optimum condition of the sel...

This study presents a new bionanocomposite coating on poly(ethylene terephthalate) (PET) made of pullulan and synthetic mica. Mica nanolayers have a very high aspect ratio (α), at levels much greater than that of conventional exfoliated clay layers (e.g., montmorillonite). A very small amount of mica (0.02 wt %, which is ϕ ≈ 0.00008) in pullulan coatings dramatically improved the oxygen barrier...

A novel thermoacidophilic pullulan-hydrolyzing enzyme (PUL) from hyperthermophilic archaeon Thermococcus kodakarensis (TK-PUL) that efficiently hydrolyzes starch under industrial conditions in the absence of any additional metal ions was cloned and characterized. TK-PUL possessed both pullulanase and α-amylase activities. The highest activities were observed at 95 to 100°C. Although the enzyme ...

Water soluble pullulan films were formatted into paper-based microfluidic devices, serving as a controlled time shutoff valve. The utility of the valve was demonstrated by a one-step, fully automatic implementation of a complex pesticide assay requiring timed, sequential exposure of an immobilized enzyme layer to separate liquid streams. Pullulan film dissolution and the capillary wicking of aq...

in the current study, whey protein- pullulan- clay nanocomposite films are prepared by casting method. the effect of nanoclay at three concentrations (1%, 3% and 5%) on physical properties such as moisture content, solubility in water, water vapor permeability and transparency of whey protein- pullulan composite films investigated. the results show that the effect of nanoparticles on composite ...

High-performance films with almost zero-birefringence and zero-wavelength dispersion were succeeded to prepare from pullulan esters derivatives (PLEs) without any additives. Optical transmittance analysis, birefringence measurement of PLE cast film and hot stretched films, and infrared dichroism analysis were conducted to characterize optical properties of PLE films comparing with cellulose tri...

Here, super-macroporous cryogel from a natural polysaccharide, pullulan was synthesized using cryo-crosslinking technique with divinyl sulfone (DVS) as crosslinker. The hydrolytic degradation of the in various simulated body fluids (pH 1.0, 7.4, and 9.0 buffer solutions) evaluated. It observed that much faster pH 9 solution than 1.0 7.4 solutions same time period. weight loss at within 28 days ...

In this study, an environmentally friendly and biodegradable pullulan/graphene bio nanocomposite was prepared coated on the nanocellulose film to improve surface, mechanical, barrier antibacterial properties. The films were by using a spray coating of suspension stainless steel plates. graphene nanoparticles modified Hummers method. nanocomposites solvent method with addition various wt% (0, 0....

Pullulanase (EC 3.2.1.41) in non-germinating seeds was compared with that in germinating seeds. Moreover, pullulanase from the endosperm of rice (Oryza sativa L., cv. Hinohikari) seeds was isolated and its properties investigated. The pI value of pullulanase from seeds after 8 days of germination was almost equal to that from non-germinating seeds, which shows that these two enzymes are the sam...