Eukaryotic protein trafficking pathways require specific transfer of cargo vesicles to different target organelles. A number of vesicle trafficking and membrane fusion components participate in this process, including various tethering factor complexes that interact with small GTPases prior to SNARE-mediated vesicle fusion. In Saccharomyces cerevisiae a protein complex of Mon1 and Ccz1 function...

Previously, a quantitative analysis that related protein synthesis inhibition of transferrin-toxin conjugates to the cellular trafficking of transferrin was proposed (P.T. Yazdi and R. M. Murphy, Cancer Res., 54: 6387-6394, 1994). Here, this work is extended to evaluate cellular trafficking of anti-transferrin receptor antibodies and protein synthesis inhibition kinetics of immunotoxins constru...

Plants recognize microbial/pathogen associated molecular patterns (MAMP/PAMP) through pattern recognition receptors (PRRs) triggering an immune response against pathogen progression. MAMP/PAMP triggered immune response requires PRR endocytosis and trafficking for proper deployment. LeEIX2 is a well-known Solanum lycopersicum RLP-PRR, able to recognize and respond to the fungal MAMP/PAMP ethylen...

Abstract Vacuolar protein sorting 45 homolog (VPS45), a member of the Sec1/Munc18 (SM) family, has been implicated in regulation endosomal trafficking. VPS45 deficiency human patients results congenital neutropenia, bone marrow fibrosis, and extramedullary renal hematopoiesis. Detailed mechanisms function are unknown. Here, we show an essential role mammalian maintaining intracellular organizat...

Previously, a quantitative analysis that related protein synthesis inhibition of transferrin-toxin conjugates to the cellular trafficking of transferrin was proposed (P. T. Yazdi and R. M. Murphy, Cancer Res., 54: 6387-6394, 1994). Here, this work is extended to evaluate cellular trafficking of anti-transferrin receptor antibodies and protein synthe sis inhibition kinetics of immunotoxins const...

The Golgi complex and the trans-Golgi network are critical cellular organelles involved in the endocytic and biosynthetic pathways of protein trafficking. Lipids have been implicated in the regulation of membrane-protein trafficking, vesicular fusion, and targeting. We have explored the role of cytosolic group IV phospholipase A(2) (cPLA(2)) in membrane-protein trafficking in kidney epithelial ...

Positive-strand RNA viruses depend on intensive manipulation of subcellular organelles and membranes to create unique viral replication (VROs), which represent the sites robust virus replication. The host endomembrane-based protein-trafficking vesicle-trafficking pathways are specifically targeted by many (+)RNA take advantage their rich resources. We summarize critical roles co-opted endoplasm...

Nigeria has been facing the immense challenge of international trafficking in persons for sexual exploitation and forced labor into countries in Europe, Asia and Africa. Being an international criminal activity driven by high profits, Nigeria's adoption of a regulatory policy is expressed in the series of legal provisions to deal with the army of traffickers. In desperation, within a space of 1...

Abstract Autophagy is an evolutionarily conserved degradation process that degrades damaged proteins to maintain homeostasis and protect cells against stress. In this study, we identified characterized a critical autophagy-related protein, UvAtg14, in Ustilaginoidea virens , which the ortholog of MoAtg14 rice blast fungus Magnaporthe oryzea . UvAtg14 co-localized with UvAtg8 (an autophagy marke...

ADP-ribosylation factors (Arf), a family of small GTP-binding proteins, play important roles in intracellular trafficking in animal and yeast cells. Here, we investigated the roles of two Arf homologs, Arf1 and Arf3 of Arabidopsis, in intracellular trafficking in plant cells. We generated dominant negative mutant forms of Arf 1 and Arf3 and examined their effect on trafficking of reporter prote...