we used various screening techniques, clustering, decision tree and generalized rule induction (association) (gri) models and molecular phylogenic relationship to search for patterns of halophi-licy and to find features contribute to halolysin salt stability. we found met was the sole n-terminal amino acid in halolysin proteins, whereas other amino acids found at this position of oth-er proteas...

introduction : soybean is an excellent plant protein source with diverse applications in food systems. despite numerous commercial applications and rich nutritional properties of soybeans, soy proteins are sensitive to heat and other damaging agents during food processing which can limit their applications in food industries. maillard reaction includes a series of chemical reactions between the...

The living cell possesses extraordinary molecular and biochemical mechanisms by which to recognize efficiently remove foreign, damaged, or denatured proteins. This essential function has been a barrier the overexpression of recombinant proteins in most expression systems. A notable exception is E. coli proteins, which, however, end-up as inclusion bodies, i.e., cytoplasmic aggregates that are i...

The biophysical properties that enable proteins to so readily evolve to perform diverse biochemical tasks are largely unknown. Here, we show that a protein's capacity to evolve is enhanced by the mutational robustness conferred by extra stability. We use simulations with model lattice proteins to demonstrate how extra stability increases evolvability by allowing a protein to accept a wider rang...

The plant hormone auxin is central to the regulation of growth and development. Recent work has demonstrated that auxin signalling depends on targeted protein degradation, and in the past year this model has been strengthened. The focus is now on identifying the targets of this degradative pathway, determining how auxin influences the degradative process and linking the turnover of specific pro...