UNLABELLED Cage-shaped protein, with its special structure, may have potential applications in biomedicine and nanotechnology. We developed a program CSPro (Cage-Shaped Protein) for efficient identification of cage-shaped proteins based on quaternary structure. CSPro is capable of revealing the corresponding cage-shaped feature more clearly and quickly than traditional visualization tools. Usin...

in protein biochemistry, biochemists specially study proteins as complex polymers. protein biochemistry is the study of the chemical structure of proteins, including individual amino acids, their linkage as well as physical structure of proteins. proteins, especially globular proteins, are three dimensional in nature. proper function of globular proteins is tightly related to their three-dimens...

Peroxidase enzymes are vastly applicable in industry and diagnosiss. Recently, we introduced a new kind of peroxidase gene from Lepidium draba (LDP). According to protein multiple sequence alignment results, LDP had 93% similarity and 88.96% identity with horseradish peroxidase C1A (HRP C1A). In the current study we employed in silico tools to determine, to which group of peroxidase enzymes LDP...

Recent studies suggest that reducing the induction temperature would improve the quality of some recombinant inclusion bodies (IB) by providing a native-like secondary structure and leading to an improvement in protein recovery. This study focused on optimizing the solubilization condition of Reteplase, a recombinant protein with 9 disulfide bonds. The influence of lowering induction temperatur...

Recent studies suggest that reducing the induction temperature would improve the quality of some recombinant inclusion bodies (IB) by providing a native-like secondary structure and leading to an improvement in protein recovery. This study focused on optimizing the solubilization condition of Reteplase, a recombinant protein with 9 disulfide bonds. The influence of lowering induction temperatur...

Protein crystallization under a magnetic field is an interesting research topic because a magnetic field may provide a special environment to acquire improved quality protein crystals. Because high-quality protein crystals are very useful in high-resolution structure determination using diffraction techniques (X-ray, neutron, and electron diffraction), research using magnetic fields in protein ...

the cytotoxic chloroform fraction of euphorbia aellenii afforded two cycloartane type triterpenes — cycloart-25-en-3,24-diol (1) and 24-methylene-cycloartan-3-ol (2) — for the first time from this plant. preparation of cycloartane derivatives, 3, 24-o-diacetyl-cycloart-25-en as compound 3 and 3-o-acetyl-24-methylene-cycloartan (4) were conducted by acetylating of 1 and 2, respectively. the ...

the tlr4 gene structure as the main receptor for lipopolysaccharide recognition of gram-negative bacteria was investigated in two strains of iranian commercial arian line and west azerbaijan native chicks and its expression was studied in some major organs. blood samples of the 120 birds were taken and total dnas extracted. then, the target gene was sequenced using four pairs of primers on four...