: World population has been continuously raised and therefore due to this fact new source of foods, particularly proteins, are in demand. In recent years, single cell protein has been considered as an accepted substitute for animal and plant proteins. In this study, single cell protein production was studied in a batch submerged culture using Aspergillus niger PTCC5012. Experimental design was ...

The group of protein drugs has been developing very strongly, promising great advances in diagnosis, treatment, prevention, and human health improvement. Protein drug production is a large area research, requiring advanced scientific technological expertise, integration multi-disciplinary technology from fields such as biology, genetics, biotechnology, extraction purification, pharmaceutical ma...

background: various prokaryotic and eukaryotic expression systems have been developed for the production of recombinant proteins. in the present study, we used a new protein expression system based on the iranian lizard leishmania, a trypanosomatid protozoan as a host, for the expression of lpg3 gene from leishmania infantum (l.infantum). methods: the lpg3 gene was cloned in the expression cass...

Cellular and tissue activities highly depend on environmental pH. Murine lung tissue, when cultured properly in-vitro, is a potent producer of hematopoietic growth factors. We have studied the effect of pH on the production of hematopoietic growth factors and protein synthesis by the murine lung in-vitro. Various concentrations of NaHC03 were used to adjust the pH of the culture medium unde...

Mammalian expression systems, due to their capacity in post-translational modification, are preferred systems for biopharmaceutical protein production. Several recombinant protein systems have been introduced to the market, most of which are under clinical development. In spite of significant improvements such as cell line engineering, introducing novel expression methods, gene silencing and pr...

an experiment was conducted to evaluate the effect of dietary crude protein and methionine levels on quality and quantity of egg production. fifteen diets formulated with 3 levels of protein (13, 14 and 15%) and 5 levels of methionine (0.25, 0.28, 0.31, 0.34 and 0.37%) and fed to 420 birds in a 3×5 factorial arrangement. each diet was randomly fed to 4 replicates of 7 birds each and fed for 3 p...

Nigeria is one of the most malnourished and hunger ridden in the league of developing countries; according to reports of Food and Agricultural Organization (FAO) of United Nations estimated daily animal protein intake for developing countries averaged at 4.5 g/head/day at the beginning of this century which has hardly risen to 10 g/head/day into the second decade of the century in Nigeria. This...

background and objective: microbial protein production can resolve one of the major world challenges, i.e. lack of protein sources. candida tropicalis growth was investigated to specify a medium to reach the highest cell proliferation and protein production. material and methods: fractional factorial design and the index of signal to noise ratio were applied for optimization of microbial protei...

Molecular farming technology offers a unique advantage that almost any protein can be produced economically and safely under very controlled conditions. Besides traditional production systems, such as bacteria, yeasts, insects and mammal cell lines, plants can now be used to produce eukaryotic recombinant proteins, especially therapeutic ones. Their advantages as hosts for protein production in...