Escherichia coli cytochrome c nitrite reductase is one of a large family of homologous enzymes that are particularly prevalent in pathogenic enterobacteria. The enzymes are periplasmic and in vivo may find themselves challenged by molecules that could enhance or compromise their performance. In the present study, we describe protein film voltammetry in which the activity of E. coli cytochrome c...

A microfluidic device that contains a silica film as optical transducer and electrochemical electrode was used to monitor how ferricyanide ion behaves within nanoporous matrix. This prototype device allows UV-VIS absorption spectroscopy and cyclic voltammetry to be performed at the same time. Significant reducing in leaching of redox molecules proved this device integrated with silica film can ...

In this work, a novel amperometric immunosensor based on multi-walled carbon nanotubes-thionine-chitosan (MWCNTs-THI-CHIT) nanocomposite film as electrode modified material was developed for the detection of chlorpyrifos residues. The nanocomposite film was dropped onto a glassy carbon electrode (GCE), and then the anti-chlorpyrifos monoclonal antibody was covalently immobilized onto the surfac...

Self-assembled multilayer films of hyaluronic acid (HA) and the protein myoglobin (Mb) were built up layer by layer on Au covered quartz crystal microbalance (AuQCM) electrode substrates. Film formation and growth were monitored by an electrochemical quartz crystal microbalance (EQCM), and the step-by-step construction was investigated through quantification of the mass variation corresponding ...

Reduced graphene oxide film was synthesized on a glassy carbon electrode by electro reduction of graphene oxide powders in aqueous solution. Then platinum nano particles were deposited on reduced graphene oxide film that was deposited on the glassy carbon electrode via electro reduction of platinum salt. The Physical morphology of the platinum on reduced graphene oxide film was evaluated by sca...

The chemolithoautotrophic bacterium NT-26 (isolated from a gold mine in the Northern Territory of Australia) is unusual in that it acquires energy by oxidizing arsenite to arsenate while most other arsenic-oxidizing organisms perform this reaction as part of a detoxification mechanism against the potentially harmful arsenite [present as As(OH)(3) at neutral pH]. The enzyme that performs this re...

A luminescent multilayer film has been fabricated from the polyoxometalate anion α[PMo12O40]3− (α-PMo12) and the cation Fuchsin Basic (FB) through an electrostatic layer-by-layer (LbL) self-assembly method and characterized by UV/Vis spectra, atomic force microscopy and X-ray photoelectron spectra. The fluorescence properties of the LbL film have also been investigated. Cyclic voltammetry measu...

this study prepared new edible composite film by blending kefiran with whey protein. the film-forming solutions used different ratios of kefiran to whey protein (70/30, 50/50, 30/70, 10/90) and were cast at room temperature. the effects of the addition of whey protein on the physical, mechanical and water-vapor permeability (wvp) properties of the film were investigated. it was found that an in...

We are investigating the redox chemistry of wild-type (WT) and mutant (1-12G) cytochrome P450 BM3. Absorption spectra in solution feature the Fe(III) Soret at 418 nm for WT and a split Soret for 1-12G at 390 and 418 nm. Voltammetry of the proteins within DDAPSS films on the surface of carbon electrodes reveal nearly identical Fe(III/II) potentials (approximately -200 mV vs Ag/AgCl), but signifi...