Glutamine carbon entry into the tricarboxylic acid (TCA) cycle was assessed in small intestinal epithelial cells by measuring CO2 production from [1-14C]glutamine, and these data together with [U-14C]glutamine data were used to calculate fractional oxidation rates for glutamine. CO2production from either [1-14C]glutamine or [U-14C]glutamine showed saturation kinetics, and the concentration need...

Wild life: The elusive fosa (Cryptoprocta ferox) is the top predator in the dry deciduous forests of Madagascar, but very little was known about its biology until the 1990s. The presence of volunteer groups studying its role in the forest’s ecology has also helped to protect its habitat. (Photo: Earthwatch/ Luke Dollar.) The world’s latest national park has just been created in the east of Russ...

This study examined the regulation of glutamine synthetase protein levels, in response to changes in external glutamine concentration, in mouse C2C12 skeletal muscle cells. Glutamine, at concentrations as low as 0.25 mmol/L, downregulated endogenous and exogenous (plasmid encoded) glutamine synthetase with maximal effect at 2 mmol/L. Glutamine appears to act by changing the stability of the glu...

  Objective(s): The purpose of the present study was to examine the effects of L-glutamine on electromyographic (EMG) activity of the quadriceps muscle injured by eccentric exercise (EE).   Materials and Methods: Seventeen healthy men (age: 22.35±2.27 yr; body mass: 69.91±9.78 kg; height: 177.08±4.32 cm) were randomly and double-blind study with subjects assigned to either an L-glutamine supple...

In enterocytes, glutamine serves as the major source of energy; another metabolic fate of glutamine is conversion to citrulline. Because sepsis can affect gut function and integrity, alterations in glutamine metabolism may exist and lead to decreased citrulline production. This study aimed to investigate how sepsis affects glutamine metabolism, including its conversion to citrulline, by measuri...

Amino acid dehydrogenases (L-amino acid: oxidoreductase deaminating EC 1.4.1.X) are members of the wider superfamily of oxidoreductases that catalyze the reversible oxidative deamination of an amino acid to its keto acid and ammonia with the concomitant reduction of either NAD+, NADP+ or FAD. These enzymes have been received much attention as biocatalysts for use in biosensors or diagnostic kit...

Glutamine transport between tissues is important for the outcome of critically ill patients. Investigation of glutamine kinetics is, therefore, necessary to understand glutamine metabolism in these patients in order to improve future intervention studies. Endogenous glutamine production can be measured by continuous infusion of a glutamine tracer, which necessitates a minimum measurement time p...

objective(s): the purpose of the present study was to examine the effects of l-glutamine on electromyographic (emg) activity of the quadriceps muscle injured by eccentric exercise (ee).   materials and methods: seventeen healthy men (age: 22.35±2.27 yr; body mass: 69.91±9.78 kg; height: 177.08±4.32 cm) were randomly and double-blind study with subjects assigned to either an l-glutamine suppleme...