Primula poissonii (Primulaceae) is a perennial herb, widely distributed in the Hengduan Mountain region of Southwest China. In this study, Roche 454 pyrosequencing was used to isolate microsatellite markers. A total of 4528 unique sequences were identified from 68,070 unique reads. Of these, eighty-seven microsatellite loci were screened for utility using two criteria: successful PCR amplificat...

Genetic diversity was investigated in 60 individuals of Primula obconica from four natural populations (from Hubei, central China, and Sichuan, south-west China) and from cultivated material. Inter-simple sequence repeat (ISSR) techniques produced 249 polymorphic bands and identified 60 ISSR genotypes. Based on Shannon's index and Nei's genetic diversity, the genetic diversity detected in all n...

Objective: The present work was undertaken to evaluate the effect of Cassia auriculata leaves extract in high fat diet induced obesity in rats. Methods: Male Wistar rats weighing 150-200 g were divided into orlistat standard, Normal control, HFD control & test groups (6 animals per group). All treatments were given orally, started after 6 weeks feeding with HFD (except normal control group) and...

In botany textbooks the farinose coating on leaves and inflorescences of many Primula species is still often said to "consist of flavone". We have shown earlier [1, 2] that it is in fact formed by varying amounts of unsubstituted flavone (at least 50%), 5-hydroxy flavone and 2'-hydroxy flavone, accompanied in many species by 5,8-dihydroxy flavone (primetin) and 5,2'-dihydroxyflavone. Further co...

The main goal of this research was to quantify the concentrations of total and ammonium N, nitrate, total and soluble P in the water in the presence of Eichhornia crassipes and Salvinia auriculata, which were submitted to two different photoperiods in a 24 hours incubation period in the laboratory. The macrophytes were incubated in plastic vials of approximately 1.5 litters, with a previously p...

Ficus palmata, Pyrus pashia and Ficus auriculata are the popular wild edible fruits of Uttarakhand. The present study aimed to evaluate their total phenolic, flavonoid contents, antioxidant and antiproliferative activities by using two solvents namely 80% aqueous methanol and acetone. The results showed highest phenolic and flavonoid contents in the Ficus palmata acetone extract, while lowest i...

Premise of the study Microsatellite primers were developed for Primula ovalifolia, a member of Primula section Petiolares (Primulaceae), to study the population genetics and species delimitation in this section. Methods and Results A total of 4753 markers were successfully designated from 5139 putative simple sequence repeat loci. We isolated 38 expressed sequence tag-simple sequence repeat m...

PREMISE OF THE STUDY Microsatellite markers from Primula sikkimensis (Primulaceae) were developed for testing deep lineage divergence and speciation events. METHODS AND RESULTS A total of 3112 microsatellites were identified from 61,755 unique reads though 454 pyrosequencing technology. Twenty-nine microsatellite loci were selected for PCR amplification and polymorphic analyses. Among the 29 ...