Precursor B-acute lymphoblastic leukemias (ALL) can be considered as malignant counterparts of normal precursor B cells arrested in a particular stage of development. So far, most studies on immunoglobulin (Ig) light chain gene rearrangements have focused on IGK-Kde rearrangements, and studies on Vκ-Jκ and Vλ-Jλ rearrangements mainly used Southern blot analysis and/or included only small series...

Southern blotting and multiple restriction enzymes were used to analyze T-cell receptor (TCR) and immunoglobulin heavy chain genes in 20 postthymic T-cell neoplasms, ten prethymic and thymic T-cell tumors, and 45 cases of precursor-B acute lymphoblastic leukemia (ALL). Immunoglobulin heavy chain, never rearranged in a postthymic specimen and only once in a prethymic/thymic sample, was rearrange...

The nucleotide sequence of gene PBI, the putative translational product of which is homologous to salivary proline-rich protein P-B, has been determined. PBI is 6.4 kb long and contains 3 exons. PBI appears to code for the precursor of a proline-rich protein which we have designated P-B1. The P-B1 precursor is composed of 134 amino acid residues including 22 residues of signal sequence, 23 resi...

Hematopoietic precursors continuously colonize the thymus where they give rise mainly to T cells, but also to B and dendritic cells. The lineage relationship between these three cell types is unclear, and it remains to be determined if precursors in the thymus are multipotent, oligopotent, or lineage restricted. Resolution of this question necessitates the determination of the clonal differenti...

We report the anti-leukemic potency of a unique biotargeted nanoscale liposomal nanoparticle (LNP) formulation of the spleen tyrosine kinase (SYK) P-site inhibitor C61. C61-loaded LNP were decorated with a murine CD19-specific monoclonal antibody directed against radiation-resistant CD19-receptor positive aggressive B-precursor acute lymphoblastic leukemia (ALL) cells. The biotargeted C61-LNP w...

A cell transfer assay system was developed to study the precursors of bone marrow-associated (B) lymphocytes in the adult mouse. The rationale of the assay is to inject into irradiated mice a cell suspension depleted of B lymphocytes, to wait a period of time to let precursor cells differentiate to B lymphocytes, then to correlate the number of B cells present in the recipient mice with the num...

In this research we report synthesis of new Barium- Cobalt precursor complex with 2,6- pyridine dicarboxylic acid (dipic).Thecomplex[Ba(H2O)6][Co(dipic)2[(1)has been characterized using spectral methods (FT-IR, UV–Vis),elemental analysis and Cyclic voltammetric (CV) method. Also in this study we report thermal decomposition of inorganic precursor complex of (1).Characterization of the binary ox...

Normal precursor B cells or hematogones share morphologic and immunophenotypic similarities with lymphoblasts of precursor B-lymphoblastic leukemia. The numbers are often increased and difficult to distinguish in many patients following chemotherapy for precursor B-lymphoblastic leukemia. The purpose of this study was to establish a unique method for differentiating hematogones from lymphoblast...