نتایج جستجو برای: precision cut tissue slices
تعداد نتایج: 1106505 فیلتر نتایج به سال:
This study is aimed at characterizing soft tissue slices using a vibratome. In particular, the effect of two sectioning parameters (i.e., step size and sectioning speed) on resultant slice thickness was investigated for fresh porcine liver as well as for paraformaldehyde-fixed (PFA-fixed) and fresh murine brain. A simple framework for embedding, sectioning and imaging the slices was established...
Infectious bronchitis virus (IBV) is an avian coronavirus affecting the respiratory tract of chickens. To analyze IBV infection of the lower respiratory tract, we applied a technique that uses precision-cut lung slices (PCLSs). This method allows infection of bronchial cells within their natural tissue composition under in vitro conditions. We demonstrate that IBV strains 4/91, Italy02, and QX ...
Renal fibrosis is a serious clinical problem resulting in the greatest need for renal replacement therapy. No adequate preventive or curative therapy is available that could be clinically used to target renal fibrosis specifically. The search for new efficacious treatment strategies is therefore warranted. Although in vitro models using homogeneous cell populations have contributed to the under...
Histological dissection of human tissue has relied on conventional procedures, which have largely remained unchanged for decades. Practices to determine measurement parameters employed in these procedures have largely relied on the use of rulers and weighing scales. It is well documented in the scientific literature that both fixation and processing of tissue can significantly affect the viabil...
Lindhe, Ö. 2001. Adrenal Bioactivation and Toxicity of 3-MeSO2-DDE, o,p ́-DDD and DMBA Investigated in Tissue Slice Culture. Acta Universitatis Upsaliensis. Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 675. 49 pp. Uppsala. ISBN 91-554-5185-3. I developed a precision-cut adrenal slice culture procedure to investigate cytochrome P450 (CYP) catalysed i...
The cryopreservation of liver slices permits optimal use of large livers and provides for the availability of the slices at any desired time. Moreover, it allows one to study liver slices from various species in the same experiment. We studied the effect of cryopreservation on the viability and metabolic activity of liver slices from rat, monkey, dog, hamster, minipig, rabbit and human. Precisi...
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