Purpose. Canaliculitis is classically associated with Actinomyces species, which are filamentous bacteria; the purpose of this study was to evaluate the extent to which nonfilamentous bacteria colonize canalicular concretions by using graded histopathological analysis. Methods. This is a series of 16 cases. The percentage of Gram-positive/Gomori's methenamine silver-positive filamentous bacteri...

We evaluated the performance of the Vitek MS for identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories. With a total of 424 well-characterized isolates, the results of the Vitek MS were compared to those of conventional methods and 16S rRNA gene sequencing. The Vitek MS correctly identified 97.9 % of the isolates tested to species level. The Vitek MS cor...

OBJECTIVES To investigate metronidazole resistance and the prevalence of nitroimidazole (nim) genes in clinically isolated anaerobic Gram-positive cocci. METHODS Metronidazole susceptibility was determined in 99 strains of anaerobic Gram-positive cocci and PCR amplification for the nim gene carried out on 61 strains (metronidazole MIC > or =0.5 mg/L). RESULTS The nimB gene was detected in 3...

In the last 30 years, the frequency, etiology, and epidemiology of bloodstream infections (BSIs) have changed with the evolution of medical care, particularly among the increasing number of hospitalized patients who require intensive care. Although gram-negative bacilli were the predominant nosocomial pathogens in the 1970s, gram-positive cocci have emerged as a more frequent cause of nosocomia...