(1983) Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis. Methods in Enzymology 91: 227. Laas T (1989) Electrophoresis in gels. In: Janson J-C and Ryden L (eds) Protein PuriTcation } Principles, High Resolution Methods and Applications. New York, Weinheim and Cambridge: VCH Publishers. Laemmli UK (1970) Cleavage of structural proteins during...

Detection of rotavirus by polyacrylamide gel electrophoresis in combination with silver staining and by enzyme-linked immunosorbent assay showed 96.7% identical results in tests with 1,304 stool specimens from diarrheic patients. The polyacrylamide gel electrophoresis method can be modified to reduce cost and working time. Phenol extraction of stools, however, is essential in maintaining the se...

A high-affinity riboflavin -binding protein was isolated and characterized for the first time from pregnant-rat sera by affinity chromatography on a lumiflavin-agarose column. The purified protein was homogeneous by the criteria of analytical polyacrylamide-gel disc electrophoresis, gel-filtration chromatography on Sephadex G-100 and sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. I...

development of a stool antigen immunoassay to detect helicobacter pylori infection requires monoclonal antibody against the specific antigen. alkylhydroperoxide reductase (ahpc) of helicobacter pylori has been described as a specific and unique enzyme for h. pylori and therefore, both h. pylori ahpc and anti-ahpc could be useful in the development of serologic and stool antigen tests, for detec...

A vitamin Blz-binding protein has been isolated from human granulocytes derived from patients with chronic granulocytic leukemia. Utilizing affinity chromatography as the sole purification technique, the protein was purified 9,860-fold with a yield of over 90% and was homogeneous based on polyacrylamide disc gel electrophoresis, sedimentation equilibrium ultracentrifugation, and sodium dodecyl ...

Alkaline phosphatase from human liver was purified to homogeneity. The purification procedure included solubilization with butanol, fractionation with acetone, and chromatography on concanavalin A-Sepharose, DEAE-cellulose, Sephadex G-200 and DEAE-Sephadex. Purity was established by standard and sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. The isoelectric point of the protein was...

A vitamin Blz-binding protein has been isolated from human granulocytes derived from patients with chronic granulocytic leukemia. Utilizing affinity chromatography as the sole purification technique, the protein was purified 9,860-fold with a yield of over 90% and was homogeneous based on polyacrylamide disc gel electrophoresis, sedimentation equilibrium ultracentrifugation, and sodium dodecyl ...