A new protocol was established for the regeneration of Solanum nigrum by frog egg-like bodies (FELBs), which are novel somatic embryogenesis (SE) structures induced from the root, stem, and leaf explants. The root, stem, and leaf explants (93.33%, 85.10%, and 100.00%, respectively) were induced to form special embryonic calli on Murashige and Skoog (MS) medium containing 1.0 mg/L 2,4-dichloroph...

Cassava brown streak disease (CBSD), caused by Cassava brown streak virus (CBSV), is an economically important disease of cassava (Manihot esculenta Crantz) in East Africa. The objective of this study was to optimise in vitro techniques for CBSV elimination from infected Ugandan cassava cultivars. Using semi-solid halfstrength Murashige and Skoog (MS) basal medium, hormone concentration and hea...

In vitro inoculation of Vitis vinifera L. cv. Chardonnay explants with a plant growth-promoting rhizobacterium, Burkholderia phytofirmans strain PsJN, increased grapevine growth and physiological activity at a low temperature. There was a relationship between endophytic bacterial colonization of the grapevine plantlets and their growth at both ambient (26 degrees C) and low (4 degrees C) temper...

the present study was undertaken to investigate the role of banana extract (be) on synchronous plantlet formation from seeds of dendrobium lituiflorum lindl. the seedlings formed on modified knudson c (kc) medium supplemented with 20% (v/v) be had significantly high fresh and dry weights. morphologically uniform plantlets with elongated leaves and well developed roots were formed on kc medium w...

To investigate the effects of salinity stress on durum wheat cultivars at critical stages germination and seedling establishment under stress, a study was conducted eight (Karim, Khiar, Inrat100, Maali, Monastir, Portodur, Razeg, Salim) subjected to an increasing concentration by using sodium chloride (NaC) (0, 2, 4, 6, 10 g L−1). Germination physiological parameters were measured thoroughly an...

Abstract The black orchid ( Coelogyne pandurata Lindley) is one of the native Indonesian species. In its natural habitat, existence threatened by continuous forest exploitation. Coveted in global market for hybridization purposes, still considered difficult to be grown cultivation and may take years flower. Its conservation breeding carried out through a combination tissue culture mutagen appli...

The African oil palm (Elaeis guineensis) is the most effective oil producer in tons per hectare. Nevertheless, its increasing cultivation in Latin America is harmed by the "lethal yellowing". Genetic resistance to this anomaly can be found in the germplasm of American oil palm or caiaué (E. oleifera), a native species from the Amazon rainforest. However, the procedures adopted to induce seeds o...

Saintpaulia ionantha Wendl. is a small flower, with variety of colors, which are commonly grown indoors for decorative purposes. Currently, micropropagation S. has been achieved certain results; however, the shoot quality as well number shoots regeneration was not high. In this study, effect LEDs system on multiplication and conducted. The leaves were cut into square patterns without main veins...

A simple and efficient protocol for recurrent somatic embryogenesis and plant regeneration is one of the prerequisites for genetic improvement of guava. An efficient reproducible regeneration somatic embryogenesis protocol was developed in four genotypes of Psidium guajava L. using immature zygotic embryo as starter explant. Somatic embryogenesis induction was obtained on MS basal medium supple...