It has been confirmed that Saccharomyces sp. W0 can produce high concentration of ethanol. In this study, the INU1 gene cloned from the marine-derived Pichia guilliermondii was transformed into uracil mutant of Saccharomyces sp. W0. The positive transformant Inu-66 obtained could produce 34.2 U ml⁻¹ of extracellular inulinase within 72 h of cultivation. It was found that 15.2 U of inulinase act...

Extracellular electron transfer (EET) of microorganisms represents a communicative bridge between the interior and exterior of the cells. Most prior EET studies have focused on Gram-negative bacteria. However, fungi and Gram-positive bacteria, that contain dense cellular walls, have rarely been reported. Herein, two model dense cell wall microorganisms (Bacillus sp. WS-XY1 and the yeast Pichia ...

Bioreduction of several prochiral carbonylic compounds such as acetophenone (1), ethyl acetoacetate (2) and ethyl phenylpropionate (3) to the corresponding optically active sec-alcohols 1a-3a was performed using wild-type strains of Pichia pastoris UBB 1500, Rhodotorula sp., and Saccharomyces cerevisiae. The reductions showed moderate to excellent conversion and high enantiomeric excess, in an ...

The aim of this study was to evaluate the effects homeopathic medicine Calcarea carbonica 12CH and bioproducts: filtrates saprobic fungi from Amazon, green propolis nosode 06CH, filtrate Pichia sp., extract, Bacillus subtilis; control Lasiodiplodia (Lasiodiplodia sp.) development passion fruit seedlings (Passiflora edulis Sims f. flavicarpa Degener). experiment carried out in a completely rando...

BACKGROUND Alicyclobacillus sp. A4 is thermoacidophilic and produces many glycoside hydrolases. An extremely acidic beta-1,4-glucanase (CelA4) has been isolated from Alicyclobacillus sp. A4 and purified. This glucanase with a molecular mass of 48.6 kDa decreases the viscosity of barley-soybean feed under simulated gastric conditions. Therefore, it has the potential to improve the nutrient bioav...

Methanol limited fed-batch cultivation was applied for production of a plant derived beta-glucosidase by Pichia pastoris. The beta-glucosidase was recovered by expanded bed adsorption chromatography applied to the whole culture broth. The new Streamline Direct HST1 adsorbent was compared with Streamline SP. Higher bead density made it possible to operate at two times higher feedstock concentrat...

Ripe banana fruit is used to isolate and identify native yeasts from the different cultivars of Musa sp. such as Champa kola, Malbhog/Sapri kola, Saiel kola, Geera kola and Jahaji kola which can be utilized for the preparation of beverages yeast. Highest mean counts of yeast were 56.3 ×103 CFU/mL in the Champa kola of Musa sp. The total 44 yeast strains were identified, comprising ascomycetes a...

Diets are a critical factor in the artificial production of lobster larvae, yet knowledge diet requirements spiny especially at its early life stages is rarely investigated. Thus, present study aimed finding potential diets larva by analyzing stomach content wild-caught ornate lobster, Panulirus ornatus, three different stages: puerulus, post-puerulus, and juvenile using eDNA metabarcoding. The...