Phytase activity was investigated in 13 lichen species using a novel assay method. The work tested the hypothesis that phytase is a component of the suite of surface-bound lichen enzymes that hydrolyse simple organic forms of phosphorus (P) and nitrogen (N) deposited onto the thallus surface. Hydrolysis of inositol hexaphosphate (InsP6 , the substrate for phytase) and appearance of lower-order ...

Consensus phytase is a new biosynthetic, heat-stable enzyme derived from the sequences of multiple homologous phytases. Two experiments were conducted to determine its effectiveness, relative to inorganic P and a mutant enzyme of Escherichia coli phytase (Mutant-EP), in improving dietary phytate-P availability to pigs. In Exp. 1, 36 pigs (3 wk old, 7.00 +/- 0.24 kg of BW) were fed a low-P corn-...

Soil samples of Bt Rhizosphere were collected from Bt cotton growing area of Andhra Pradesh, India and was used as a source material for isolation and screening of phytase producing bacteria. 19Bacteria were isolated from Bt Rhizosphere. Phytase enzyme activity of the cultures was screened on modified phytase solubulizing medium (MPSM). The result inferred that six isolates BtRS1 to BtRS6 were ...

PurposeOrganic anions commonly released from plant roots and microorganisms are widely reported to mobilize soil phosphorus (P). We characterized organic P that was mobilized by assessed its amenability hydrolysis phosphatase enzymes.MethodsSix soils differing in concentration were extracted with citrate, malate or oxalate solutions incubated preparations of phosphomonoesterase, phosphodiestera...

Objective: A study was conducted to determine the effect of phytase inclusion in broiler breeder diets on fecal and egg characteristics of individually caged females. Materials and Methods: A total of 184 female broiler breeders were fed growing and laying diets containing 0.7% or 2.7% calcium (Ca) and 0.35% or 0.12% available phosphorus (AvP), respectively, with one of four graded levels of ph...

The 1074-bp phyCs gene (optimized phyC gene) encoding neutral phytase was designed and synthesized according to the methylotrophic yeast Pichia pastoris codon usage bias without altering the protein sequence. The expression vector, pP9K-phyCs, was linearized and transformed in P. pastoris. The yield of total extracellular phytase activity was 17.6 U/ml induced in Buffered Methanol-complex Mediu...

Phytate, the major source of phosphorus in seeds, exists as a complex with different metal ions. Alkaline phytases are known to dephosphorylate phytate complexed with calcium ions in contrast to acid phytases that act only on phytic acid. A recombinant alkaline phytase from Bacillus sp. MD2 has been purified and characterized with respect to the effect of divalent metal ions on the enzyme activ...

this study was conducted to evaluate the effects ofcitric acid and phytase supplementation on performance, blood lipid profile, immune system and some carcass characteristics of broiler chickens.for this purpose 240, one-day old male broiler chicks were reared throughout 42 day production, in factorial experiment 2×2, including citric acid (0 and 1%) and phytase enzyme (0 and 500 ftu) based on ...

Enzymes can catalyze various reactions with high selectivity and are involved in many important biological processes. However, the general instability of enzymes against high temperature often limits their application. To address this, we synthesized a trehalose-based hydrogel in two steps from commercial starting materials with minimal purification procedures. Mono- and multi-functional trehal...

Techniques have been developed to produce microbial phytase for addition to diets for simple-stomached animals, with the aim to improve phosphorus availability from phytate-P in plant sources. The activity of the crude microbial phytase showed pH optima at pH 5.5 and 2.5. The enzyme was able to degrade phytate in vitro in soya-bean meal, maize and a liquid compound feed for pigs. When microbial...