Quasi-elastic neutron scattering (QENS) and small angle (SANS), in combination with isotopic contrast variation, have been used to determine the structure dynamics of three-component lipid membranes, form vesicles, comprising an unsaturated [palmitoyl-oleoyl-phosphatidylcholine (POPC) or dioleoyl-phosphatidylcholine (DOPC)], a saturated phospholipid (dipalmitoyl-phosphatidylcholine (DPPC)), cho...

INTRODUCTION Phosphatidylcholine and phosphatidylcholine-derived metabolites exhibit anti-inflammatory properties in various stress conditions. We hypothesized that dietary phosphatidylcholine may potentially function as an anti-inflammatory substance and may decrease inflammatory activation in a chronic murine model of rheumatoid arthritis (collagen-induced arthritis). METHODS The experiment...

Multilamellar phosphatidylcholine liposomes (0.1 pmol) and 1 : 1 phosphatidylcholinecholesterol multilamellar liposomes (0.094pmol) were incubated with 2 x lo6 unstimulated CBA mouse peritoneal macrophages in Fisher's medium. A, Phosphatidylcholine liposomes+macrophages ; 0, phosphatidylcholine liposomes alone; A, phosphatidylcholins-cholesterol liposomes+maaophages; 0, phosphatidylcholin~holes...

The content and composition of classes molecular species phospholipid extracted from Dalbergia tonkinensis Prain (Cây Sưa) seed oil in Viet Nam have been investigated using HPLC-HRMS LCMS-IT-TOF. subclasses their D. for the first time. results showed that contains all basic lipid: polar lipid, sterol, triacylglycerol, free fatty acid, diacylglycerol, hydrocarbon, wax. In which, triacylglycerol ...

the effects of camp-analogue dibutyryl-camp and anticalmodulin w-7 were studied on de novo synthesis and secretion of lipids in cultures of hepatoma mcardle rh7777 cells and normal rat hepatocytes. dibutyryl-camp and w -7 separately caused a significant decrease in the secretion of de novo synthesized triacyl [3h]glycerol in both cultures of mcardle cells and rat hepatocytes. the inhibitory eff...

A bile salt (sodium taurodeoxycholate, NaTDC) was used to prevent phase separation between silica and lipid in self-assembled long-chain diacyl phosphatidylcholine/SiO(2) films. Phase diagrams for NaTDC/didecanoyl phosphatidylcholine/SiO(2) and NaTDC/egg phosphatidylcholine/SiO(2) films were investigated through grazing-incidence small-angle X-ray scattering at a synchrotron source.

1. Phenobarbitone injection did not affect the concentration of phospholipids in the liver endoplasmic reticulum, but it increased the rate of incorporation of [(32)P]orthophosphate into the phospholipids. 20-Methylcholanthrene caused a transient increase in total phospholipid but a decrease in the turnover rate of the phospholipids. 2. Incorporation of [(32)P]orthophosphate into phosphatidylch...

The Saccharomyces cerevisiae opi3-3 mutant was shown to be defective in the synthesis of phosphatidylcholine via methylation of phosphatidylethanolamine. The opi3-3 mutant was isolated on the basis of an inositol excretion phenotype and was not auxotrophic for choline. Inositol, but not choline, stimulated growth of the mutant. The opi3-3 mutation was recessive and was genetically linked to the...

Chlorohydrins of stearoyl-oleoyl phosphatidylcholine (SOPC), stearoyl-linoleoyl phosphatidylcholine, and stearoyl-arachidonyl phosphatidylcholine were incubated with cultured myeloid cells (HL60) for 24 h, and the cellular ATP level was measured using a bioluminescent assay. The chlorohydrins caused significant depletion of cellular ATP in the range 10-100 microM. The ATP depletion by the phosp...

The HL-60 leukemia cell line derived from a human acute promyelocytic leukemia is stimulated to differentiate into macrophages within 24-28 hr after exposure to the phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA). We studied early alterations (within 90 min of exposure to TPA) in phosphatidylcholine metabolism in HL-60 cells and found that phosphatidylcholine synthesis by methylation ...