Little is known about the prevalence, functionality and ecological roles of temperate phages for members of the mycolic acid producing bacteria, the Mycolata. While many lytic phages infective for these organisms have been isolated, and assessed for their suitability for use as biological control agents of activated sludge foaming, no studies have investigated how temperate phages might be indu...

A two dimensional electrophoretic method is described for the routine clinical analysis of urinary proteins. Cellulose acetate electrophoresis is used for the first dimension, and SDS (sodium dodecyl sulphate) electrophoresis for the second dimension, the latter being performed together with gel staining (Coomassie Blue) on the „Phast System". The Separation media are supplied äs "ready-to-use"...

The standard PCR technique allows the rapid detection of microorganisms, difficult to detect by traditional culture methods. However, the control of samples through PCR has the drawback of false positives due to amplification of DNA/RNA of non-viable cells. This can be overcome by adding intercalating agents (EMA or PMA) to the sample, which selectively penetrates nonviable cells and blocks the...

Increasing scarcity of fossil fuels makes the deployment of hydrogen in combination with renewable energy sources, nuclear energy or the utilization of electricity from full time operation of existing power stations an interesting alternative. A pre-requisite is, however, that the safety of the required infrastructure is investigated and that its design is made such that the associated risk is ...

Phaeobacter arcticus Zhang et al. 2008 belongs to the marine Roseobacter clade whose members are phylogenetically and physiologically diverse. In contrast to the type species of this genus, Phaeobacter gallaeciensis, which is well characterized, relatively little is known about the characteristics of P. arcticus. Here, we describe the features of this organism including the annotated high-quali...

Germline mutations in exons 10, 11, and 16 of the RET protooncogene are associated with the heritable cancer syndromes multiple endocrine neoplasia (MEN) type 2A, familial medullary thyroid carcinoma (FMTC), and MEN type 2B. Nonradioactive mutation analysis with nondenaturing Phastgels and the Phast System was performed on DNA amplified by the polymerase chain reaction from exons 10, 11, and 16...