Single-use bioreactors are commonly used for seeding stainless steel bioreactors or for producing material. The profitability of these equipments has been well demonstrated on more that decade. But few data on its scalability have been published. In 2010-2011, Merck Serono Biodevelopment performed a study in order to evaluate the performance of several disposable bioreactors. As different techn...

The Saccharomyces cerevisiae centrosome or spindle pole body (SPB) is a dynamic structure that is remodeled in a cell cycle dependent manner. The SPB increases in size late in the cell cycle and during most cell cycle arrests and exchanges components during G1/S. We identified proteins involved in the remodeling process using a strain in which SPB remodeling is conditionally induced. This strai...

Duplication of the yeast centrosome (called the spindle pole body, SPB) is thought to occur through a series of discrete steps that culminate in insertion of the new SPB into the nuclear envelope (NE). To better understand this process, we developed a novel two-color structured illumination microscopy with single-particle averaging (SPA-SIM) approach to study the localization of all 18 SPB comp...

Abstract El Niño-Southern Oscillation (ENSO) asymmetry in predictability on springtime initial condition remains unclear. From the perspective of spring barrier (SPB), this paper investigates ENSO SPB and explores potential factors that may lead to asymmetry. Both observation 29 Coupled Model Intercomparison Project Phase 6 (CMIP6) models show sea surface temperature (SST) persistence is signif...

This study evaluates associations between soil lead concentrations (SPb), age of housing, and blood lead levels (BPb) of children in metropolitan New Orleans and Lafourche Parish, Louisiana. The database includes over 2,600 SPb and 6,000 BPb samples paired by their median values and pre-1940 housing percentages for 172 census tracts. Associations were evaluated with Fisher's exact test and Spea...

Mps1 is a conserved kinase that in budding yeast functions in duplication of the spindle pole body (SPB), spindle checkpoint activation, and kinetochore biorientation. The identity of Mps1 targets and the subdomains that convey specificity remain largely unexplored. Using a novel combination of systematic deletion analysis and chemical biology, we identified two regions within the N terminus of...

Dendroctonus fi-ontaiis Zimm. (southern pine beetle (SPB)) has caused over $900 million in damage to pines in the southern United States between 1960 and 1990 (Price et al.. 1992). The damage of SPB to loblolly (Pinus tuedu L.), shortleaf (Pinus echinata Mill.), and pitch (Pinus rigida Mill.) pine has long been established (Hopkins. 1899), however, extensive mapping of SPB infestations has only...

We use small-angle x-ray scattering (SAXS) as a tool to study the binding of proteins to spherical polyelectrolyte brushes (SPB) in situ. The SPB consists of a solid core of approximately 100 nm diam onto which long polyelectrolyte chains [poly(styrene sulfonic acid, PSS) and poly(acrylic acid, PAA)] have been densely grafted. The proteins used in this investigation, Bovine Serum Albumine (BSA)...

Introduction Single-Use Bioreactors are now commonly used for Process Development activities, as seeding bioreactors or to produce Drug Substances. The advantages of this equipment have been well demonstrated over the last years on batch/fed-batch processes. Continuous processes were widely applied in the past to increase the overall productivity of small bioreactors or for sensitive molecule p...