OBJECTIVES This review discusses challenges to stability, analytics and manufacturing of protein coformulations. Furthermore, general considerations to be taken into account for the pharmaceutical development of coformulated protein drug products are highlighted. KEY FINDINGS Coformulation of two or more active substances in one single dosage form has recently seen increasing use offering sev...

Small-angle neutron scattering was employed to study protein crowding under freezing conditions that mimic those used in pharmaceutical processing. The results demonstrate that, although there is an increase in heterogeneity as the temperature is reduced, sorbitol reduces protein crowding in both solution and freeze-concentrated phases, thus protecting the protein from forming oligomers or irre...

Small-angle scattering (SAS) is a versatile analytical technique that provides detailed structural information from sample at the length scale of nanometers. In this overview, we discuss basic principles SAS and diverse applications in characterization pharmaceutical materials. Examples include identification liquid-crystalline mesophase drugs, influence excipient microstructure on final produc...

Date palm seed (Phoenix dactylifera) (DPS) extract has been used worldwide in pharmaceutical agent due to its constituents. This study aimed to assess the effects of DPS extract as a dietary supplementation at on the body composition, lipid peroxidation and tissue quality of common carp (Cyprinus carpio) based on the total volatile nitrogen (TVN) test. Common carp juveniles weighing ca 32 g wer...

A lectin-like protein of unknown function designated as LSMT was recently discovered in the edible mushroom Agaricus bisporus. The protein shares high structural similarity to HA-33 from Clostridium botulinum (HA33) and Ricin-B-like lectin from the mushroom Clitocybe nebularis (CNL), which have been developed as drug carrier and anti-cancer, respectively. These homologous proteins display the a...

Protein production for structural and biophysical studies, functional assays, biomarkers, mechanistic studies in vitro and in vivo, but also for therapeutic applications in pharma, biotech and academia has evolved into a mature discipline in recent years. Due to the increased emphasis on biopharmaceuticals, the growing demand for proteins used for structural and biophysical studies, the impact ...

Background: Parathyroid Hormone (PTH) is secreted by parathyroid glands and controls the level of calcium in bones and kidney. PTH is a small polypeptide with 84 amino acids, but the first 34 amino acids of which are enough for hormone biological activity and can be used in the treatment of Osteoporosis. The expression efficiency of recombinant human parathyroid hormone rhPTH (1-34) or Teripara...

Natural pharmaceutical ingredients have been widely used in recent decades due to their safety and biocompatibility. Zein, a plant-derived natural protein, has several advantages over other synthetic polymers bone tissue engineering (BTE). This study of zein protein focuses more on its application BTE as potential biopolymer material scaffold development. The use shown benefits the production s...

Bone marrow stromal stem cells (BMSCs) play a significant role in cell therapy. These cells quickly die after transplantation to the affected area due to oxidative stress. The natural disaccharide, trehalose which can be known as autophagy inducer. The present study aimed to investigate the role of trehalose in preventing BMSCs from oxidative stress caused by H2O2. BMSCs were isolated from the ...