Recently we described (Electrophoresis 5: 143-147, 1984) a new device for producing density and solute gradients, involving computer-controlled coöperation of stepmotor-driven high-precision burettes, the purpose being to substantially improve reproducibility, flexibility, and documentation of gradients used in gels and in other applications in biochemistry. Here we present the functional princ...

Plant group classification relation to soil environmental characteristics was monitored in Hangzhou Bay coastal wetland, China. 18 taxa, belonging to 17 genus and 7 family were recorded, and classified into five groups by TWINSPAN viz A, Scirpus mariqueter group, B, Spartina alterniflora group, C, Phragmites communis group, D, Tamarix chinensis P. communis group, and E, Salix matsudana Imperata...

Transport mechanisms for NaCl in intestinal brush border membrane vesicles (BBMV) of herbivorous, seawater-adapted tilapia, Oreochromis mossambicus, were investigated with a rapid filtration technique using 22Na+ and 36Cl- as tracers. Evidence to support the existence of cotransport mechanisms (Na+/Cl- or Na+/K+/2Cl-) in tilapia intestinal BBMV was not found. An inwardly directed Cl- gradient o...

Coenzyme A (CoA) transport was studied in isolated rat heart mitochondria. Uptake of CoA was assayed by determining [3H]CoA associated with mitochondria under various conditions. Various oxidizable substrates including alpha-ketoglutarate, succinate, or malate stimulated CoA uptake. The membrane proton (delta pH) and electrical (delta psi) gradients, which dissipated with time in the absence of...

Electrokinetic flow of a suspension of erythrocytes (red blood cells, RBCs) in 20 num cylindrical fused-silica capillaries is examined in the present work. Flow direction anomalies are observed experimentally and tentatively explained by the development of a pH gradient between the cathode well and the anode well due to electrolysis reactions at the electrodes. This pH gradient alters the local...