An in vitro transcription system using nuclei from freely suspended callus cells of Petroselinum crispum is described. The use of a filtration technique allowed the measurement of transcription and release of RNA simultaneously over very short time intervals. The transcription showed a biphasic time course, with an early maximum at 2 and a later one at 20 minutes. The early maximum was ascribed...

In this study we evaluated the antibacterial activity of the crude hydroalcoholic extracts, fractions, and compounds of two plant species, namely Rosmarinus officinalis and Petroselinum crispum, against the bacteria that cause urinary tract infection. The microdilution method was used for determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). T...

Cells o f Petroselinum crispum cv. “Paramount” and “Plain-leaved” immobilized in polyure­ thane foam retained the biotransformational characteristics o f their freely suspended cells for certain monoterpenic substrates. Immobilized cells o f both cultivars were more efficient than their free cells in isomerizing geraniol into nerol, but less efficient (<60% ) in reducing the alde­ hydes, citral...

The timing o f changes in the protein synthesis pattern o f elicitor-treated, [35S]methioninelabelled parsley cells ( Petroselinum crispum) was analyzed by two-dimensional gel electropho­ resis. Five groups were distinguished from a large number o f elicitor-responsive as well as un­ responsive proteins. Two groups were synthesized de novo either early or late after elicitor ap­ plication; two ...

Two NADPH:cytochrome P450 oxidoreductases (CPRs) from parsley (Petroselinum crispum) were cloned, and the complete proteins were expressed and functionally identified in yeast. The two enzymes, designated CPR1 and CPR2, are 80% identical in amino acid sequence with one another and about 75% identical with CPRs from several other plant species. The mRNA accumulation patterns for CPR1 and CPR2 in...

Some plants contain various compounds, which can influence microbial growth, reproduction or basic cell functions. Phenols, polyphenols, micronutrients, essential oils and other compounds belong to them. These compounds are primarily present in various herbs. Extracts from these plants could be used as natural food preservatives, which could inhibit growth of undesirable microorganisms. In this...