Selective transformations of limonene by asco- and basidiomycetes were investigated. On the shake flask scale, Penicillium citrinum hydrated R-(+)-limonene to a-terpineol [83% regioselectivity (rs), more than 80 mg l(-1) product yield], and Gongronella butleri catalysed the terminal oxidation to yield perillyl alcohol (60% rs, 16 mg l(-1)). On the laboratory bioreactor scale, Penicillium digita...

Post-harvest diseases ,caused by Penicillium digitatum and Penicillium italicum, lead to considerable losses of citrus fruits. Several research studies have focused on biological properties and antimicrobial activity of essential oils (EO) extracted from the Medicinal and Aromatic Plants (MAP). The main objective of this paper is to study the antifungal effects of two essential oils of the fami...

Endophytic fungi live in inter-cellular spaces of healthy plant tissues without causing any apparent symptoms diseases for the host plant. Some fungal endophytes help their hosts to survive under biotic and abiotic stresses. In this study, we collected mango leaves at Honghe plantations (Yunnan Province) winter. A total 34 different endophytic strains were isolated, phylogenetic placements esti...

Penicillium digitatum is the most destructive postharvest pathogen of citrus fruits, causing fruit decay and economic loss. Additionally, control of the disease is further complicated by the emergence of drug-resistant strains due to the extensive use of triazole antifungal drugs. In this work, an orthologus gene encoding a putative sterol regulatory element-binding protein (SREBP) was identifi...

Peroxynitrite is an important chemical in the human immune system, which has high biocidal activity and can resist invasion of pathogens. Currently, vitro generation peroxynitrite faces major technological challenges, especially for gas-phase production, requires low-pressure conditions. Here, we report method how to generate at atmospheric pressure using dielectric barrier discharge. Results s...

Stored citrus fruit suffer huge losses because of the development of green mould caused by Penicillium digitatum. Usually synthetic fungicides are employed to control this disease, but their use is facing some obstacles, such public concern about possible adverse effects on human and environmental health and the development of resistant pathogen populations. In the present study quercetin, scop...

Characterization of the phosphate effect on ethylene production by Penicillium digitatum is reported. A low level of phosphate (0.001 millimolar) was about 200 to 500 times as effective as a high phosphate level (100 millimolar) in stimulating ethylene production and the stimulation was readily reversed by addition of phosphate. This phosphate effect did not operate in static cultures. The prec...

The small antimicrobial peptide PAF26 (Ac-RKKWFW-NH(2)) has been identified by a combinatorial approach and shows preferential activity toward filamentous fungi. In this work, we investigated the mode of action and inhibitory effects of PAF26 on the fungus Penicillium digitatum. The dye Sytox Green was used to demonstrate that PAF26 induced cell permeation. However, microscopic observations sho...

The sterol regulatory element binding proteins (SREBPs) are key regulators for sterol homeostasis in most fungi. In the citrus postharvest pathogen Penicillium digitatum, the SREBP homolog is required for fungicide resistance and regulation of CYP51 expression. In this study, we identified another SREBP transcription factor PdSreB in P. digitatum, and the biological functions of both SREBPs wer...