an enzyme-linked immunosorbent assay for neopterin using penicillinase as marker enzyme is reported here by polyclonal antibodies against neopterin conjugated to bovine serum albumin which were raised in rabbits. immunoglobulin fractions were purified and coated on wells of microtiter plates. a chain heterology was introduced in neopterin derivative and conjugated to penicillinase. the assay is...

A conjugation system initially discovered in beta-lactamase-producing gonococci mobilized small non-selftransmissible R plasmids encoding beta-lactamase (penicillinase) production into other gonococci, Neisseria, and Escherichia coli. This conjugation system was mediated by a separate selftransmissible plasmid of 23.9 X 10(6) daltons, pFA2. Conjugative plasmids capable of mobilizing R plasmids ...

Yip, Lily C. (University of Cincinnati, Cincinnati, Ohio), Ramesh Shah, and Richard A. Day. Metabolic control of penicillinase biosynthesis in Bacillus cereus. J. Bacteriol. 88:297-308. 1964.-Penicillinase production in strains 5 and 5/B of Bacillus cereus in response to treatment by 6-aminopenicillanic acid (APA), penicillin G, (6-N-alpha-(p-benzyloxyphenoxy)-propionylamino-penicillanic acid, ...

serum cortisol level was measured by an enzyme immunoassay (eia) using the enzyme penicillinase as a label without prior extraction and purification. polyclonal antibodies were raised against cortisol-3-ortho-carboxymethyl-oxime (cortisol-3-0-cmo) conjugated to bovine serum albumin (bsa). this antibody showed a very low cross-reactivity with structurally related steroids (3.7% for corticosteron...

nowadays immunochemical techniques have played a very important and valuable role in quantitative and qualitative assays of liquid compounds of the body. producing antibody against immunogenes is the first step to make immunochemical kits. in this study production and purification of polyclonal antibody against melatonin has been considered. this hormone which has several important functions in...

An inducible penicillinase was shown to be present in a strain of Aeromonas hydrophila subsp. hydrophila isolated from freshwater fish. Enzyme induction was observed with benzylpenicillin or 6-aminopenicillanic acid, and the enzyme was cell bound. The penicillinase was purified 50-fold from a crude cell extract. The molecular weight was estimated to be 23,000 by gel filtration. The pH and tempe...

Only 30-50 yo of the cell-bound penicillinase of Bacillus cereus NRRL 569 is neutralized by antiserum prepared against the exo-penicillinase. The unneutralizable fraction is not decreased by cell disintegration which liberates a proportion of the cell-bound enzyme into solution. Absence of neutralization cannot therefore be explained by the existence of a mechanical barrier which prevents acces...

Since penicillinase-producing Neisseria gonorrhoeae appeared five years ago in West Africa and South-east Asia reported cases have doubled annually in Great Britain, primarily as a result of increasing importation. Importation of penicillinase-producing Neisseria gonorrhoeae has increased exponentially because dramatic expansion of these strains in their regions of origin has led to increasing ...

Of 53 strains of Neisseria gonorrhoeae isolated in Benin City, Nigeria, in February 1983 to October 1984, 46 (87%) produced penicillinase. The minimum inhibitory concentrations (MICs) of penicillin G and ampicillin for these isolates were between 1 mg/l and and 50 mg/l. About 48% (22/46) of the penicillinase producing strains were also resistant to streptomycin, cotrimoxazole, and ampicillin an...