BACKGROUND Feline immunodeficiency virus (FIV) infection is an important cause of disease of cats worldwide. Initial screening is commonly performed by commercially available point-of-care (POC) ELISA tests. Confirmatory testing for positive POC test results is recommended. Polymerase chain reaction (PCR) tests for FIV are commonly used additional testing methods; however, reported measures of ...

BACKGROUND Efficacy of Helicobacter pylori stool antigen enzyme immunoassay (HpSA) and stool PCR was evaluated, before and after treatment, in a country with a high prevalence of H. pylori infection. METHODOLOGY A total of 52 patients with dyspeptic symptoms were included in the study. Antral biopsy was collected during pre- and post-therapy periods for rapid urease test (RUT) and PCR. Simila...

Introduction and Aim: Polymerase chain reaction (PCR) of 16S rRNA virulence genes associated with Type Three Secretion System (TTSS) has been used as a rapid method for the identification pathogen Enterobacter cloacae in leukemia patients. Rapid diagnosis this becomes necessary starting proper treatment these In current study, we aimed to isolate from patients study TSSS isolates using molecula...

BACKGROUND We noted a marked increase in Chlamydia trachomatis (CT) infections in the Capital Health Region of NS coincident with substitution of a PCR for an enzyme immunoassay (EIA). We reviewed our experience to determine the cost of switching and the impact on the number of new infections diagnosed. METHODS Information on the number of EIA and PCR tests performed on women was retrieved fr...

Objective : The study aimed to evaluate molecular and immunological methods propose a workflow using them for tuberculosis (TB) diagnosis routine. Methods A cross-sectional retrospective was performed, including 121 liquid cultures from TB laboratory located in the extreme south of Brazil. All were positive Mycobacterium complex (MTBC) by in-house Polymerase Chain Reaction (PCR) DNA extracted C...

Aim: One of the important methods to detect SARS CoV-2 is polymerase chain reaction (PCR) during pandemic outbreak. There are a number real time PCR tests, which recommended from World Health Organization (WHO). Usually, WHO test should give correct and reproducible results. Material Methods: The tests conducted perform comparisons between for N gene ready use kit FR475 Genekam Biotechnology AG...