Objective(s) Amongst the various antibiotic resistant elements in Vibrio. cholerae, SXT constin (SXT-C) is important. We were going to design a quick method for determination of antibiotic resistance gene pattern in SXT-C. Materials and Methods Ninety four V. cholerae O1El Tor isolates were used in this study. Antibiotic susceptibility testing, multiplex PCR amplification of SXT-C containing...

PURPOSE Early identification of causative agents in lower respiratory infection of pediatric patients can reduce morbidity and prevent an overuse of antimicrobials. Two kinds of multiplex polymerase chain reaction (PCR) and a commercial shell vial viral culture were performed to identify causative agents in pediatric patients. MATERIALS AND METHODS Nasopharyngeal aspirates of 220 children dia...

Multiple infections by pathogens are currently the most serious problems in pig herds. Clinically, accurate diagnosis is difficult due to similarity of the symptoms of porcine pseudorabies virus (PRV), porcine parvovirus (PPV), and porcine circovirus type 2 (PCV2). A multiplex polymerase chain reaction (multiplex-PCR) was developed and optimized for the simultaneous detection of the three DNA v...

BACKGROUND A cost-effective, accurate and rapid simultaneous multiplex assay is required for testing and diagnoses of conventional and emerging viruses in clinical virology laboratories. We developed and optimized a dual priming oligonucleotide (DPO) multiplex PCR assay for detecting influenza viruses including seasonal H1N1, 2009 pandemic H1N1, H3N2, influenza B and H5N1. METHODS The optimiz...

Salmonella (S.) Typhimurium and S. Enteritidis are the major causative agents of food-borne illnesses worldwide. Currently, a rapid detection system using multiplex real-time polymerase chain reaction (PCR) has been applied for other food-borne pathogens such as Escherichia coli, Staphylococcus aureus and Streptococcus spp. A multiplex real-time PCR was developed for the simultaneous detection ...

Foot-and-mouth disease virus (FMDV), Bluetongue virus (BTV), Vesicular stomatitis Virus (VSV), Bovine viral diarrheal (BVDV), Bovine rotavirus (BRV), and Bovine herpesvirus 1 (IBRV) are common cattle infectious viruses that cause a great economic loss every year in many parts of the world. A rapid and high-throughput GenomeLab Gene Expression Profiler (GeXP) analyzer-based multiplex PCR assay w...

background and objectives: streptococcus pneumoniae is the most common cause of invasive infections among both young children and elderly people. common serotypes causing invasive diseases and the emergence of carriers of streptococcus pneumoniae in iran is not yet known. past-vaccine surveillance studies of serotype prevalence patterns in iran are necessary to monitor the epidemiology of strep...