نتایج جستجو برای: pcr gene detection
تعداد نتایج: 1737497 فیلتر نتایج به سال:
3,4- Methylenedioxymethamphetamine (MDMA or "Ecstasy") is a psychoactive and hallucinogenic drug of abuse. MDMA has been shown to produce neurotoxicity both in animals and humans. Recently, the vasodilator drugs such as pentoxifylline is one of the new strategies which have been considered as neuroprotector. In this study effect of pentoxifylline on bcl-2 gene expression changes in hippocampus ...
Background and Objective: Methicillin resistance in Staphylococcus aureus is an increasingly important clinical problem. A chromosomal gene, mecA, mediates resistance to penicillinase-resistant penicillins such as methicillin and oxacillin in Staphylococcus aureus. We evaluated the validity of disk diffusion test by using oxacillin, methicillin an...
avian influenza virus (aiv) infection is a major cause of bird or human mortality and morbidity, therefore the rapid identification of the virus is of important clinical and epidemiological implication. methods: a multiplex reverse transcriptase pcr (rt-pcr) was optimized for the detection of influenza a virus and the h5 and h9 subtypes. the influenza type a specific primers were directed to th...
scid disorder is major failure of the immune system, usually genetic. the aim of this study was on mutations detection of rag1, rag2, and il7rg genes in scid cases. mutation detection was performed by pcr sequencing. our results indicated that 13 mutations were found through cases which include 4 mutations in il7r gene (t661i, i138v, t56a, c57w), 7 mutations in rag1 (w896x, w204r, m324v,...
Determination of RT-PCR detection limit of live and dead Salmonella cells in raw and sterilized milk
The objective of the current study was to evaluate the reproducibility of a reverse transcriptase PCR (RT-PCR)-based technique to differentiate viable and dead Salmonella cells in raw and sterilized milk. The microorganism was initially inoculated into the milk samples followed by incubating at 37°C for 4 h prior to inactivation by heat at 80°C for 10 min. The treated and non-treated samples we...
Background and purpose: Molecular detection of antibiotic resistance in clinical strains of M.tuberculosis is of great importance. In this study, we developed a method for rapid detection of mutations resistant to the rifabutin antibiotic resistant gene. Material and methods: In this study 40 clinical isolates of M.tuberculosis including 12 resistant and 28 susceptible isolates to rifabutin we...
3,4- Methylenedioxymethamphetamine (MDMA or "Ecstasy") is a psychoactive and hallucinogenic drug of abuse. MDMA has been shown to produce neurotoxicity both in animals and humans. Recently, the vasodilator drugs such as pentoxifylline is one of the new strategies which have been considered as neuroprotector. In this study effect of pentoxifylline on bcl-2 gene expression changes in hippocampus ...
influenza a virus is now considered to be widespread in poultry and has demonstrated the ability to infect humans in iran. for laboratory diagnosis of these respiratory viruses, it is essential to have rapid methods, able to detect viruses in early stages of the infection in clinical specimens. the real-time reverse-transcription polymerase chain reaction n (rrt-pcr) assay has been established ...
background: the diagnosis of ocular toxoplasmosis is mainly based on clinical features. however, ocular fluid testing by pcr may be very helpful for approval or rejection of this etiology. in this study, we utilized a nested-pcr technique, targeting the b1 partial sequence to analyze the aqueous and vitreous samples for evaluating the presence of the toxoplasma dna. methods: fifty aqueous or vi...
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