We demonstrated recently that in Chinese hamster ovary cells stably expressing human recombinant endothelinA receptors (CHO-ETAR), endothelin-1 (ET-1) activates two types of Ca 2 permeable nonselective cation channels (designated NSCC-1 and NSCC-2) and a store-operated Ca channel (SOCC), which can be distinguished by Ca channel blockers such as 1-{ -[3-(4-methoxyphenyl)propoxy]-4-methoxyphenyle...

UNLABELLED Antibodies against donor's HLA antigens and B cell activity are recognized modulators of immune response to allograft. The role of both anti-HLA and non-HLA antibodies is understood in solid organ transplantation, but has not been addressed in composite tissue allografts. AIM We decided to evaluate the presence and role of anti-HLA and non-HLA antibodies after hand transplantation....

Human papillomaviruses (HPV) are associated with cervical cancer and interact with growth factors that may enhance malignant transformation of cervical carcinoma cells. Endothelin-1 (ET-1) is released from HPV transfected keratinocytes and induces increased growth response in these cell lines in comparison with normal cells. In the present study several cervical carcinoma cell lines have been a...

Overexpression of endothelin (ET)-1 and its receptors, ETAR and ETBR, commonly referred to as the 'ET-axis', has been demonstrated to play a role in cancer progression for various human tumours. Based on these results we propose a similar role of the expression of the ET-axis in vulvar cancer. Expression of the ET-axis was investigated immunohistochemically using tissue microarrays with tumour ...

Evidence lacks on the differential effects of the two therapeutic concepts of endothelin receptor antagonists (ERA): the blockade of only the endothelin-receptorA (ETAR) (selective antagonism) versus both ETAR and ETBR (dual blockade). Ambrisentan, a selective ERA, and bosentan, a dual blocker, are both available for therapy. We hypothesized that there are differences in the potential of ERAs t...

The advent of targeted therapeutics in human cancer has begun to find novel druggable targets and, in this context, the endothelin-1 receptor (ET-1R), namely ETA receptor (ETAR) and ETB receptor, among the GPCR family represents a class of highly druggable molecules in cancer. ET-1R are aberrantly expressed in human malignancies, potentially representing prognostic factors. Their activation by ...

Objective: As a distinct cellular signaling model, receptor crosstalk between G protein-coupled receptors (GPCR) and epidermal growth factor (EGFR) has been demonstrated in various vitro studies. In addition, recent studies had focused on the pathways of endothelin-1 (ET-1) pathophysiology cancer. Accordingly, growing interest analysis crosstalk ET-1 EGFR functional consequences activation pro...

In order to become established in the skeleton, metastatic cells disseminating from the breast carcinoma need to acquire organ-specific traits. There are no effective predictors for who will develop bone metastasis to guide long-term predictive therapy. Our purpose was to individuate events critical for bone colonization to make a molecular classification of breast carcinoma useful for bone-met...

Introduction: Each treatment planning algorithm has some errors in dose calculation. Particularly these errors are more pronounced in the heterogeneous regions. It is recommended for each set of radiation data and algorithm subtle deliberation done regarding dose calculation accuracy. Knowing the amount of error in dose calculation will result in a fairly accurate estimate of t...