Abstract Background The generation of accurate and reproducible viral sequence data is necessary to understand the diversity present in populations RNA viruses isolated from clinical samples. While various sequencing methods are available, they often require high quality templates titer ensure reliable data. Methods We modified a multiplex PCR approach characterize simian immunodeficiency virus...

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission has been reported worldwide and novel SARS-CoV-2 variants continue to emerge. A strain, the Delta variant (B.1.617.2), is spreading worldwide. The reportedly high infectivity immune evasion potency. In June 2021, World Health Organization categorized it as a of concern (VOC). Therefore, vital develop tests that can exclus...

BACKGROUND Previous findings regarding risk factors for human papillomavirus (HPV) persistence, other than viral determinants, identified from prospective cohort studies have been inconsistent in part because study designs have differed with respect to differing HPV detection methods and varying lengths of follow-up time. Therefore, the objectives of this study were to continue the search for e...

The purpose of this study was to compare hybrid capture assay with PCRs using different primers for the L1, E6-E7 regions for the detection of human papillomavirus (HPV) genome. One hundred twenty-five cervical smears with normal (n=42) and abnormal (n=83) cytology were investigated. Those at high-risk for HPV were studied by hybridization antibody capture assay and PCR with the pU-1M/pU-2R pri...

A rapid real-time multiplex PCR assay for detecting and differentiating Bordetella pertussis and Bordetella parapertussis in nasopharyngeal swabs was developed. This assay (LC-PCR-IS) targets the insertion sequences IS481 and IS1001 of B. pertussis and B. parapertussis, respectively, and is performed using the LightCycler (Roche Molecular Biochemicals, Indianapolis, Ind.). The analytical sensit...

Carbapenemase producing carbapenem-resistant Enterobacteriaceae (CP-CRE) are an epidemiologically important emerging public health threat. Early and reliable detection of CP-CRE is for containment these pathogens. The aim this study was to evaluate observe the sensitivity polymerase chain reaction (PCR)-dipstick DNA chromatography (GeneFields CPE) carbapenemase genes (NDM, OXA-48, KPC, IMP) in ...

miR-499a-5p has been reported to regulate the progression of various tumours. However, role in breast cancer is unclear. The purpose this study was investigate and mechanism cancer. growth effect on cells investigated by CCK-8 assay, wound healing assay Transwell invasion assay. luciferase activity used verify downstream targets miR-499a-5p. levels GSH, MDA ROS were detected kits. Quantitative ...

Human papillomavirus (HPV) infection is the leading risk factor for cervical intraepithelial neoplasia (CIN) and cervical cancer. Detection of high-risk HPV infections might identify women who are at increased risk of development or progression of a cervical lesion and may have prognostic significance. Therefore there is considerable interest in identifying and accurately typing the viruses. PC...