Jumlah penderita diabetes melitus meningkatkan setiap tahunnya sehingga kebutuhan insulin meningkat. Insulin merupakan obat utama untuk pasien mellitus tipe 1 (DMT1). Dalam beberapa kasus, 2 (DMT2) memerlukan pengobatannya. Saat ini, Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris, dan Hansenula polymorpha sistem ekspresi yang digunakan secara luas memproduksi prekursor insulin. Pre...

objective: organophosphorus hydrolase (oph) is a homodimeric enzyme that can hydrolyze phosphoester bonds and reduce the toxicity of organophosphorus compounds. this makes oph a suitable element for the biodegradation of these compounds. methods: we successfully cloned the oph gene from pseudomonas diminuta, after optimization for pichia pastoris, into a yeast expression vector (ppiczαb). afte...

introduction: follicle stimulating hormone (fsh) is one of the pituitary glycoproteines that it consists of two subunits alpha and beta. the beta subunit is responsible for the biological activity of fsh. the aim of present study was isolation of the β subunit coding sequence containing its signal sequence from human genome and then cloning of the isolated sequence in ppic9 shuttle vector under...

To express the antimicrobial peptide cecropin D in Pichia pastoris and determine the activity of the expressed product, four oligonucleotide fragments were synthesized in accordance with the available cecropin D sequences and a codon bias suitable for Pichia pastoris. Sequence fragments were phosphorylated, annealed, linked and cloned into the expression vector pGAPZαA and the yeast α-mating fa...

BACKGROUND Pichia pastoris is one of the most important host organisms for the recombinant production of proteins in industrial biotechnology. To date, strain specific parameters, which are needed to set up feeding profiles for fed batch cultivations, are determined by time-consuming continuous cultures or consecutive fed batch cultivations, operated at different parameter sets. RESULTS Here,...

Laccase is a copper-containing polyphenol oxidase that has great potential in industrial and biotechnological applications. Previous research has suggested that fungal laccase may be involved in the defense against oxidative stress, but there is little direct evidence supporting this hypothesis, and the mechanism by which laccase protects cells from oxidative stress also remains unclear. Here, ...