Type II DNA topoisomerases permit passages of double stranded DNA segments through each other and this is achieved via a complex mechanism involving a transient cleavage of one duplex, a passage of the second duplex through the topoisomerase-spanned cleavage site and finally resealing of the cut duplex. Type II DNA topoisomerases facilitate many DNA transactions requiring manipulation of long D...

Directed graphs can be partitioned in so-called passages. A passage P is a set of edges such that any two edges sharing the same initial vertex or sharing the same terminal vertex are both inside P or are both outside of P . Passages were first identified in the context of process mining where they are used to successfully decompose process discovery and conformance checking problems. In this a...

BACKGROUND Limited resources for adult stem cells necessitate their in vitro culture prior to clinical use. Investigating mitochondrial DNA (mtDNA) and telomere shortening has proved to be important indications of stem cell validity. This study was designed to investigate these indicators in multiple passages of three adult stem cell lines which were produced in our stem cell laboratory. METH...

Objective(s) The main objective of this study was to investigate the status of chromosome stability in 3 human-mouse hybridoma cell lines over a period of time in various passages. Materials and Methods Metaphase spreads from 3 human-mouse cell lines (HF2X653, SPMO-4 and F3B6) that had been cultured in 4 successive passages, from 1 to 4 weeks, were prepared and analyzed. Metaphase chromosome...