In Paper I of this series (1) a method was described for the paper chromatographic analysis of certain ketonic steroids as hydrazones formed with Girard’s Reagent T. When that technique was applied to the adrenal cortical hormones, however, certain deficiencies appeared. (a) The RI values of the individual steroid-Reagent T hydrazones were not sufficiently different to permit identification. (b...

The example of column chromatography (Figure 1) demonstrates the typical features found in this analytical technique. The diagram shows an experiment where a two-component mixture is subjected to column chromatography. The column is packed with a solid material called the stationary phase. A liquid solvent or eluting solution is poured into the column and completely wets the solid packing mater...

Paper chromatography is an analytical technique for separating and identifying mixtures of materials. It is a useful technique since it is relatively quick, and requires small quantities of the material. By emergence of nanotechnology, paper chromatography has found many applications in biology and biotechnology. In this study, we employed gold and silver nanoparticles to detect protein bands a...

paper chromatography is an analytical technique for separating and identifying mixtures of materials. it is a useful technique since it is relatively quick, and requires small quantities of the material. by emergence of nanotechnology, paper chromatography has found many applications in biology and biotechnology. in this study, we employed gold and silver nanoparticles to detect protein bands a...