Rice stripe virus (RSV) is a plant virus, and its infection leads to devastating disease that often causes serious yield losses in rice-producing regions. In this study, the capsid protein (CP) gene of RSV was cloned expressed E. coli (Rosetta), purified recombinant His-CP used immunize New Zealand white rabbits . After six weeks, polyclonal antibody against (PAb-CP ) obtained from serum immuni...

BACKGROUND Banding of the pulmonary artery (PAB) in preparation for arterial switch operation (ASO) in patients with transposition of the great arteries (TGA) represents a unique model of acute left ventricular pressure overload in humans. METHODS AND RESULTS To establish the rate, magnitude, and determinants of left ventricular hypertrophy and the acute effect on ventricular function, serial...

The rate of occurrence and magnitude of provoked augmented breath (PAB) were studied as the function of lung expansion applied at different intervals (15-180 s). Together with phrenic nerve activity (Phr.) the activities of recurrent laryngeal (RL) and hypoglossal (Hyp) nerves were investigated during PAB. The experiments were carried out in 10 cats anesthetised, paralysed and artificially vent...

Antibodies to DNA are important markers of various autoimmune diseases and can be pathogenic; however, their generation is not understood. We previously reported that anti-DNA antibodies could be induced in mice by idiotypic immunization to PAb-421, an antibody to a DNA-binding domain of p53. We now report that two monoclonal antibodies of moderate affinity (K(D) asymptotically equal to 10(-7))...

BACKGROUND A unique clade of the bacterium Mycoplasma gallisepticum (MG), which causes chronic respiratory disease in poultry, has resulted in annual epidemics of conjunctivitis in North American house finches since the 1990s. Currently, few immunological tools have been validated for this songbird species. Interleukin-1β (IL-1β) is a prototypic multifunctional cytokine and can affect almost ev...

A new species of spiroplasma, Spiroplasma eriocheiris (S. eriocheiris), was identified as a lethal pathogen of tremor disease (TD) in Chinese mitten crab recently. In order to acquire appropriate biological and diagnostic tools for characterizing this newly discovered pathogen, 5 monoclonal antibodies (mAbs) and a polyclonal antibody (pAb) against S. eriocheiris were produced. Among the mAbs, 6...

INTRODUCTION Military parachuting has been shown to result in injuries. This investigation systematically reviewed studies examining the influence of the parachute ankle brace (PAB) on injuries during military parachuting and performed a cost-effectiveness analysis. EVIDENCE ACQUISITION Parachute ankle brace studies were obtained from seven databases, personal contacts, and other sources. Inv...

background & aim: the most common primary brain tumors of the central nervous system are gliomas. among a number of different biomolecular events, a strong relation between oxidative stress pathways and the development of this cancer has been proved. oxidative stress (os) is the consequence of an imbalance between pro-oxidants and antioxidants towards pro-oxidants. the pro-oxidants cause lipid ...

An experimental condition was established in vivo for selectively eliminating hapten-reactive suppressor T-cell activity generated in mice primed with a para-azobenzoate (PAB)-mouse gamma globulin (MGG)-conjugate and treated with PAB-nonimmunogenic copolymer of D-amino acids (D- glutamic acid and D-lysine; D-GL). The elimination of suppressor T-cell activity with PAB-D-GL treatment from the mix...

l h e poly(A) tail of eukaryotic mRNAs associates with poly(A)binding (PAB) proteins whose role in mRNA translation and stabiiity is being intensively investigated. Very little is known about the structure and function of the PAB genes in plants. We have cloned multiple PAB-related sequences from Arabidopsis thaliana. Results suggest that PAB proteins are encoded by a multigene family. One memb...