mixed malaria infections, plasmodium falciparum and p. vivax, are suspected to occur at a greater frequency than is detected by conventional light microscopy. in order to determine the year round pattern of transmission and the frequency of mixed infections in malaria endemic area, we carried out a prospective comparison of diagnosis by conventional light microscopy and nested pcr in chahbahar ...

BACKGROUND Outside of Africa, P. falciparum and P. vivax usually coexist. In such co-endemic regions, successful malaria control programs have a greater impact on reducing falciparum malaria, resulting in P. vivax becoming the predominant species of infection. Adding to the challenges of elimination, the dormant liver stage complicates efforts to monitor the impact of ongoing interventions agai...

We have compared Duffy blood group genotype distribution, as determined by polymerase chain reaction with allele-specific primers, in 68 Plasmodium vivax-infected patients and 59 non-vivax malaria controls from Rondônia, Brazil. Homozygosity for the allele Fy, which abolishes Duffy antigen expression on erythrocytes, was observed in 12% non-vivax controls but in no P. vivax patient. However, no...

BACKGROUND Evidence of the presence of Plasmodium vivax in the placenta is scarce and inconclusive. This information is relevant to understanding whether P. vivax affects placental function and how it may contribute to poor pregnancy outcomes. METHODS Histopathologic examination of placental biopsies from 80 Papua New Guinean pregnant women was combined with quantitative polymerase chain reac...

To determine the level of antimalarial drug resistance in southern Papua, Indonesia, we assessed the therapeutic efficacy of chloroquine plus sulfadoxine-pyrimethamine (CQ+SP) for Plasmodium falciparum infections as well as CQ monotherapy for P. vivax infections. Patients with P. falciparum failing therapy were re-treated with unsupervised quinine+/-doxycycline therapy and those with P. vivax w...

background: plasmodium vivax is responsible for approximately 80 million malaria cases in the world. apical membrane antigen1 (ama-1) is a type i integral membrane protein present in all plasmo­dium species. ama-1 interferes in critical steps of invasion of human hepatocytes by sporo­zoites and red blood cells by merozoites and is one of the most immunodominant antigens for elicit­ing a protect...

background: the present study was formulated in order to determine pol­ymorphism of dihydropteroate synthetase gene (dhps) of plasmodium vivax (p . vivax ) in hormozgan province, southern iran and mutations at codons 382, 383, 512, 553, and 585 associated with resistance of p. vivax to sulfadoxine. method : one-hundred eighteen isolates of p. vivax were prepared within 2007-2008 to determine di...

Hybridomas producing monoclonal antibodies (MAbs) directed against P. vivax erythrocytic stage antigens were isolated. Here, we describe the production of six MAbs. Culture supernatant from growing hybridomas was tested for their reactivity with P. vivax erythrocytic stages by ELISA against P. vivax crude (PvC) antigen and by immunofluorescence assay on P. vivax infected erythrocyte smears. P. ...

Approximately 55% of malaria infections in the Guyana Amazon region are attributed to Plasmodium falciparum while the other 45% are attributed to non-falciparum, mostly Plasmodium vivax. However, little is known about the P. vivax strain types circulating in the region. Using PCR for Plasmodium detection and two genetic markers specific to P. vivax to detect the polymorphic circumsporozoite pro...

Currently the local P. vivax was sharply decreased while the imported vivax malaria increased in China. Despite Southeast Asia was still the main import source of vivax malaria, the trend of Africa become serious, especially for west and central Africa. Herein we have clarified the trend of P. vivax in China from 2004-2012, and made some analysis for the differences of imported vivax back from ...