We tried to develop an experimental system for quantifying piroplasm transmission in Theileria sergenti infection using a SCID mouse model, of which red blood cells (RBC) had been substituted with bovine (Bo). A mouse hybridoma cell producing antibody against Holstein RBC was established for distinguishing Holstein RBC from Japanese Black RBC by a immuno-staining method. T. sergenti piroplasms ...

b a ckground: cutaneous leishmaniasis due to leishmania major is a serious and increasing problem affecting manyrural areas of 17 out of 31 provinces in iran. little is known about sand fly fauna and leishmaniases in eastern iran and no study has been carried out in sarbisheh county. the aim of this study was to determine sand flies composi- tion and probable leishmania infection to find the pr...

A 32 kilodalton major piroplasm surface protein (MPSP) is expressed abundantly on the surface of intraerythrocytic piroplasms of Theileria sergenti and is considered to be a candidate antigen for vaccine development against piroplasmosis. In this study, transcripts of MPSP gene were detected in an expression cDNA library prepared from T. sergenti-infected tick salivary glands. Expression of MPS...

The OFF! Clip-On fan vaporizer device releasing metofluthrin was evaluated against phletobomine sand flies in the Judean Desert, Israel, in October, 2009. A total of 76,400 sand flies was collected, with male flies representing 98.3%Phlebotomus sergenti and 1.7%P. papatasi. Females comprised 43.0% of the total catch and included 6.7% blood-fed females. Similar proportions of flies were collecte...

a disease of the dry desert area where phlebotomus papatasii p. sergenti are prevalent. In India cases of oriental sore are prevalent in the dry western zone (western part, 79? east of Greenwitch) where the insect vector is phlebotomies papatasii; on the other hand cases of kala-azar are seen in the eastern humid zone where p. argentipes is the insect vector responsible for the spread of the di...

In Morocco, as in most countries around the Mediterranean, leishmaniasis is an important public health problem. The situation has become worrying since the 1970’s and continues to be more complicated. The objective of this work was to realize a historical overview of the classification, evolution, and dispersion of Leishmania parasites and Sandflies in Morocco. The status of the different leish...

A polymorphism in the experimentally successful peptide vaccine sequence (EVVWKEKKEVKDLDA, amino acids 134-148) derived from the 33 kDa piroplasm major surface antigen (p33) of Theileria sergenti was examined. The vaccine sequences obtained by PCR amplification and sequencing of the p33 gene from a total of 15 parasite-infected cattle blood samples collected from 4 prefectures through Hokkaido ...