In the mammalian ovary, progressive activation of primordial follicles serves as the source of fertilizable ova, and disorders in the development of primordial follicles lead to various ovarian diseases. However, very little is known about the developmental dynamics of primordial follicles under physiological conditions, and the fates of distinct populations of primordial follicles also remain ...

Campylobacter and Salmonella are known to cause acute bacterial gastroenteritis in humans. Raw poultry products have been implicated as a significant source of these infections. Five trials were conducted to determine whether Campylobacter and Salmonella spp. exist naturally in the mature and immature ovarian follicles of late-life broiler breeder hens. Broiler breeder hens ranging from 60 to 6...

background: cryopreservation of mammalian ovaries has been reported with different levels of success. cryopreservation of ovarian tissue may be a potential alternative for treatment of infertility and many attempts have been done to improve the efficiency of ovarian cryopreservation. the objective of the present study was to compare the direct cover vitrification (dcv) with ethylene glycol (eg)...

Three experiments were conducted to evaluate factors affecting number of surface ovarian follicles and oocytes yield and quality in buffalo. In Experiment 1, ovaries (n = 126) were collected in pairs from slaughtered anoestrus, early pregnant and cyclic buffaloes. Ovarian follicles (1-3, 4-9 and > or = 10 mm diameter) were counted, aspirated and oocytes were recovered and evaluated. In Experime...

OBJECTIVE To evaluate the effects of freezing and thawing on the histologic changes in ovarian fragments from sheep and to determine the feasibility of ovarian autografts. DESIGN Histologic evaluation of follicles that survived after freezing at -196 degrees C for 2 weeks. Histologic evaluation of ovarian fragments 6 months after the autografts. SETTING Laboratoire de Zootechnie, Ecole Nati...

When doses of progestin used commercially are administered to synchronize the stage of the estrous cycle among bovine females, fertility is reduced compared with that of untreated controls. The reduced fertility that results from the use of progestin based estrus synchrony regimens is associated with the development of persistent ovarian follicles. Persistent ovarian follicles develop as a resu...

Apoptosis is a mode of physiological cell death that occurs during tissue regression and remodeling; it is characterized by macromolecular protein synthesis, oligonucleosomal fragmentation, nuclear condensation, cytoplasmic shrinkage, and dissociation of cell-to-cell junctional complexes. Direct in situ fluorescence detection of digoxigenin end-labeled genomic DNA was used as a marker of nuclea...

Background: With all the recent advances in cancer treatments, many young cancer patients find themselves facing the prospect of losing their fertility after aggressive chemotherapy or radiotherapy. Cryopreservation of ovarian cortical tissue has emerged as a potential option to restore fertility in these young women. Materials and Methods: Because autotransplantation of cryopreserved ovarian c...

Ovarian follicles are a major site of oestrogen synthesis in sheep, but almost nothing is known about the relative contribution made by follicles of different sizes to the output of oestrogen at various stages of the reproductive cycle. To determine this, ovaries were dissected from sheep, all follicles over 2 mm in diameter were explanted separately as organ cultures and the daily production o...