Diphosphoglycerate mutase (DPGM) was purified to homogeneity from human erythrocytes. The enzyme and Freund adjuvant were infected into chickens and yielded a monospecific precipitating antibody. Radial immunodiffusion with this antibody was used to measure the amount of DPGM in hemolysates from human adult and cord red cells. Dog, rabbit, rat, chicken, and goat red cells all had DPGM during th...

Production of group A streptococcal pyrogenic exotoxins (SPE) type A and C was transferred from toxigenic streptococcal strains to nontoxigenic strains by lysogeny. Lysogens were tested for SPE with Ouchterlony immunodiffusion on Todd-Hewitt agar plates; toxin diffusing from isolated colonies reacted with specific hyperimmune antisera to SPE. Phage prepared from strains T25(3) (T12gl) and 3GL16...

We investigate pattern avoidance in alternating permutations and generalizations thereof. First, we study pattern avoidance in an alternating analogue of Young diagrams. In particular, we extend Babson-West’s notion of shape-Wilf equivalence to apply to alternating permutations and so generalize results of Backelin-West-Xin and Ouchterlony to alternating permutations. Second, we study pattern a...

Normal human serum contains 18 +/-5 mg/100 ml of C1 esterase inhibitor (alpha-2 neuraminoglycoprotein) as estimated by immunochemical means. Of 118 patients with hereditary angioneurotic edema, the sera of 80, from 42 kindred, contained a mean concentration of 3.15 mg/100 ml or 17.5% of normal. The mean serum concentration in 35 patients in 7 other kindred was 20 mg/100 ml or 111% of normal, an...

SHIRACHI , R., SLTKENO, N., UMMENAT, T. and ISRIDA, N. Heavy-density Particles of Australia Antigen Detected in a Human Plasma Pool. Tohoku J. exp. Med., 1972, 107 (4), 359-365„ŸBiophysical study of Australia antigen (Au) was made with 5 specimens including 3 plasmas and 2 plasma pools which had been known to be Au carriers. A fraction which could be detected both by comple ment fixation test a...

Antigens recognized by anti-gamma-seminoprotein (gamma-Sm) antibody and anti-prostate-specific antigen (PA) antibody were analyzed immunologically by Ouchterlony method and Western blotting method. The double immunodiffusion test demonstrated that anti-gamma-Sm antibody and anti-PA antibody reacted against an identical antigen in seminal fluid or prostate homogenate. In the immunoblotting exami...