In general, mammalian males produce only spermatozoa in their testes and females produce only oocytes in their ovaries. However, newborn MRL/MpJ male mice produce oocytes within their testes. In this study, we examined the initiation and progression of oogenesis in fetal and neonatal MRL/MpJ mouse testes and evaluated the characteristics of testicular oocytes. Germ cells with positive reactions...

The orb gene encodes an RNA recognition motif (RRM)-type RNA-binding protein that is a member of the cytoplasmic polyadenylation element binding protein (CPEB) family of translational regulators. Early in oogenesis, orb is required for the formation and initial differentiation of the egg chamber, while later in oogenesis it functions in the determination of the dorsoventral (DV) and anteroposte...

The Bicaudal-D (Bic-D) gene is required early in Drosophila oogenesis for the differentiation of an oocyte from one of a cluster of 16 interconnected germarial cells. To analyze the role of Bic-D later in oogenesis, we have constructed Drosophila lines in which Bic-D expression is under the control of the hsp70 promoter. In these flies, Bic-D activity can be induced early in oogenesis, allowing...

Reconstituting gametogenesis in vitro is a key goal for reproductive biology and regenerative medicine. Successful in vitro reconstitution of primordial germ cells and spermatogenesis has recently had a significant effect in the field. However, recapitulation of oogenesis in vitro remains unachieved. Here we demonstrate the first reconstitution, to our knowledge, of the entire process of mammal...

During Drosophila melanogaster oogenesis, spindle assembly occurs without centrosomes and relies on signals from chromosomes. Beaven et al. (2017. J. Cell. Biol. https://doi.org/10.1083/jcb.201704120) show that 14-3-3 proteins bind and inhibit a key microtubule motor, Ncd, during oogenesis, but Aurora B releases Ncd inhibition near chromosomes, allowing Ncd to work in the right time and place.

The Deleted in Azoospermia gene family encodes three germ cell-specific RNA-binding proteins (DAZ, DAZL and BOLL) that are essential for gametogenesis in diverse species. Targeted disruption of Boll in mice causes male-specific spermiogenic defects, but females are apparently fertile. Overexpression of human BOLL promotes the derivation of germ cell-like cells from genetically female (XX), but ...

abstract increase in the use of nano zinc oxide in medicine, manufacturing cosmetic products and research, have raised the questions about their toxicity. zinc has direct role in growth, maturation and ovulation through influence on production of estradiol and progesterone. in this study the effects of nano zinc oxide on oogenesis of adult nmri mouse strain have been investigated. the adult fem...

In order to investigate the origin and spatial distribution of maternal mRNA during oogenesis, in situ hybridization with [3H]-poly(U) was utilized for the detection of poly(A)-containing RNA [poly(A)+RNA] in histological sections of Oncopeltus fasciatus ovaries. In the germarium poly(A)+RNA was found to accumulate in the trophocyte cytoplasm concomitant with the maturation of these cells. Poly...

Cyclin J (CycJ) is a poorly characterized member of the Cyclin superfamily of cyclin-dependent kinase regulators, many of which regulate the cell cycle or transcription. Although CycJ is conserved in metazoans its cellular function has not been identified and no mutant defects have been described. In Drosophila, CycJ transcript is present primarily in ovaries and very early embryos, suggesting ...

Background and Objective: Spermatogenesis and oogenesis stress is a primary epigenetic element that can cause lifelong modifications in neural system functions. This study was designed to elucidate whether preconceptional stress during spermatogenesis and oogenesis affects the seizure behaviors and body weight of the offspring later in life. Materials and Methods: In this experimental study, 1...