Background: Successful in vitro maturation (IVM) of oocytes is an important step for production of livestock through assisted reproductive techniques including somatic cell nuclear transfer (SCNT). Obtaining an optimal duration for maturation of oocyte is essential, since over exposure to maturation medium results in oocyte aging, while under exposure leads to production of immature incompetent...

developing a culture system for preantral follicles has important biotechnological implications due to the potential to produce a large number of oocytes for embryo production and transfer. to accomplish this goal, the present study was aimed to culture preantral follicles in the presence of different media, sera and fsh concentrations. six-week-old preantral follicles (95 ± 5 µm) were cultured...

Electron microscope examination of thin sections of bullfrog (Rana catesbeiana) ovarian oocytes has shown the presence of mitochondria containing yolk-crystal inclusions in oocytes of all sizes, from 160 to 1500 micro mean diameter. The hexagonally shaped yolk-crystals have major periodicities of 73.8 +/- 10.7 A (n = 100). Several forms of modified mitochondria, observed in the smaller oocytes,...

An estimate has been made of the numbers of mitochondria in the mitochondrial cloud (Balbiani body) of Xenopus laevis oocytes ranging in size from 50 to 250 micrometers. The mitochondrial number is expressed in terms of a 'standard' organelle measuring 2 micrometers in length and 0.2 micrometer in diameter and is derived by measurements on electron micrographs of sections through the cloud. It ...

Electron microscope examination of thin sections of bullfrog (Rana catesbeiana) ovarian oocytes has shown the presence of mitochondria containing yolk-crystal inclusions in oocytes of all sizes, from 160 to 1500 μ mean diameter . The hexagonally shaped yolk-crystals have major periodicities of 73 .8 f 10.7 A (n = 100) . Several forms of modified mitochondria, observed in the smaller oocytes, ma...

This study combines microfluidics with optical microablation in a microscopy system that allows for high-throughput manipulation of oocytes, automated media exchange, and long-term oocyte observation. The microfluidic component of the system transports oocytes from an inlet port into multiple flow channels. Within each channel, oocytes are confined against a microfluidic barrier using a steady ...

Abstract In Masbate, senatorial scallop Chlamys senatoria is one of the five commercially important species present in Asid Gulf, but its basic biology poorly understood Philippines. To provide biological information for future conservation and management, reproductive rhythm relation to lunar phase was investigated from October 2016 March 2017. Scallops were collected daily fishers’ catches me...

BACKGROUND Previous studies showed that immature oocytes stored in ovarian tissue could develop to the mature stage after transplantation. However, the quality and competency of the oocytes developed in xenografted ovarian tissue have never been investigated. As a pilot study to investigate this uncharted issue, we evaluated microtubule organization and chromatin configuration of human oocytes ...

To determine the optimum culture duration for porcine growing oocytes (GOs) to attain maturation competence, we examined the meiotic competence, chromatin configuration, and fertilization ability of porcine oocytes obtained from early antral follicles and cultured for 10-16 days. The survival rate of oocytes after 10 days of culture (62.8%) was similar to that of oocytes after 12 days of cultur...