Objective To assess the effect of fibronectin (Fn) and porcine type I collagen (PCOL) on odontoblast-like cells in vitro. Material and Methods Rat odontoblast-like cells (MDPC-23 cells) were inoculated and cultured on Fn-coated or type I collagen-coated substrates. Proliferation assay, alkaline phosphatase activity (ALP activity), mRNA expression of hard tissue-forming markers, and Alizarin r...

Runx2 is an essential transcription factor for bone and tooth development whose function in odontoblast differentiation remains to be clarified. To pursue this issue, we examined tooth development in Runx2 transgenic mice under the control of Col1a1 promoter (Tg(Col1a1-Runx2) mice). Endogenous Runx2 protein was detected in the nuclei of preodontoblasts, immature odontoblasts, mesenchymal cells ...

Monkeys were perfused with Karnovsky phosphate-buffered formalin-glutaraldehyde (PBFGTA). Molars were removed and postfixed in PBF-GTA, demineralized in EDTA, freeze-fractured, and critical-point dried. Odontoblast processes were observed in tubules of the predentine, the pulpal inner third of dentine and in the peripheral dentine, but not in the middle third. Peripheral processes showed close ...

Quantitative evaluation of autoradiographic grains from anoxia-treated and control neonatal hamster indicated that anoxia impairs collagen synthesis and significantly suppresses the incorporation of proline-'H in the odontoblast, fibroblast, and osteoblast by 24 hours after the insult. However, the rapid and apparently total recovery of the dental and paradental connective tissue cells suggests...