Interferon regulatory factor 7 (IRF7) gene was cloned from a subtractive cDNA library constructed with mRNAs obtained from rainbow trout (Oncorhynchus mykiss) macrophage cell line (RTS-11). Using expressed sequence tag clones of submitted IRF7 amino acid sequences, specific primers were designed. Results showed that IRF7 cDNA contains an ORF of 1251 nucleotides that translates into a 416 resi...

Human peripheral blood monocytes have been found to undergo a transitory state of high accessory activity before they fully become macrophages. Time kinetics were done to follow this accessory potential. Studying the regulation of accessory activity, we have found that monocyte derived accessory cells (m-AC) pass through two phases of development, both of which are adversely controlled by ...

Cationic calix[6]arene and calix[4]arene derivatives non-covalently bind into a DNA minor groove or RNA major form tweezer-like complexes with nucleotides.

Genome sequencing is the process of determining in which order nitrogenous bases also known as nucleotides within a DNA molecule are arranged. Every organism’s genome consists unique sequence nucleotides. These provide phenotypes and genotypes cell. In mathematics, Graph theory study mathematical objects graphs made vertices (or nodes) connected by either directed edges or indirect edges. Deter...

1. Base: Also referred to as heterocycles, these nitrogencontaining ring compounds are derivatives of purine or pyrimidine. The atoms within the purine/pyrimidine rings do not vary and they are given the same number in different nucleotides. A variety of chemical groups can be bonded at different positions to the ring constituents.Althoughone structure predominates for each base, tautomeric for...

Molecular chaperones such as heat shock protein 70 (Hsp70) are crucial for protein folding. Crystal structures of Hsp70 in a complex with the nucleotide exchange factor (NEF) Hsp110 reported in this issue of Cell (Polier et al., 2008) and in Molecular Cell (Schuermann et al., 2008) provide new insights into how NEF action specifies Hsp70 cellular function.

n vertebrate cells, not all potential DNA replication origins fire in each S phase, but it is unclear what parameters control that decision. Mauro Anglana, Michelle Debatisse (Institut Curie, Paris, France), and colleagues find that nucleotide availability can determine which origins are activated. Under normal conditions (when nucleotides were readily available), initiation occurred predominan...