It has been demonstrated (1, 2) that dietary guanine is not utilized to a significant extent by the rat for nucleic acid synthesis but that adenine may be utilized by the rat as a precursor of both polynucleotide adenine and polynucleotide guanine (2). Recent studies with labeled glycine (3) and nucleic acid (4) have indicated that polynucleotide guanine may also arise by mechanisms not involvi...

A new, general preparation of 2'-5'-linked oligoadenylates has been developed, which relies on: (1) chemoselective 0phosphorylation of N-unblocked nucleosides via hydroxyl activation by Grignard reagents, (2) one-pot construction of internucleotide linkage using bifunctional phosphorylating agents, and (3) selective production of a 3',5'-di-O-protected adenosine. This solution-phase synthesis a...

There is considerable attention directed at chemically modifying nucleic acids with robust functional groups in order to alter their properties. Since the breakthrough of copper-assisted azide-alkyne cycloadditions (CuAAC), there have been several reports describing the synthesis and properties of novel triazole-modified nucleic acid derivatives for potential downstream DNA- and RNA-based appli...

The formation of regenerating wound tissue involves not only production of new cells but also synthesis of relatively large amounts of protein (l-5). From these facts, it may be inferred that while this new tissue is being formed, nucleic acid metabolism is probably different from that observed in normal animals. There has been some indication that this may be the situation for ribonucleic acid...

The formation of regenerating wound tissue involves not only production of new cells but also synthesis of relatively large amounts of protein (l-5). From these facts, it may be inferred that while this new tissue is being formed, nucleic acid metabolism is probably different from that observed in normal animals. There has been some indication that this may be the situation for ribonucleic acid...

Although many investigations concerning the content of nucleic acids in bacterial cells have been reported (Stacey, 1948; Belozersky, 1947; Boivin, 1948), no systematic study of nucleic acid synthesis during growth was undertaken until the studies of Malmgren and his co-workers (1945, 1947a,b,c,d,e) and of Cohen (1948). The results of the study of Malmgren and co-workers showed that the total n...