and patterns of expression, γ-crystallins have generally appeared to be the most lens-specific of the crystallins. Here we examine the mouse γS-crystallin (γS) gene and its expression. Methods: The cDNA and gene for mouse γS were cloned and sequenced. The Crygs gene was mapped using genetic crosses. Expression patterns in mouse and cow were examined by northern blot, PCR and western blot using ...

A cDNA clone of Eucommia ulmoides Oliv. encoding auxin binding protein 1 (ABP1), one of the putative receptors of auxin, was isolated, and the seasonal expression of ABP1 in relation to IAA and ABA annual variation was investigated by different technical approaches including RT-PCR, real-time PCR, northern blotting, western blotting, and immunolocalization. In the cambial region, ABP1 expressio...

We present a modeling study of the long-range transport of pollution from Europe, showing that European emissions regularly elevate surface ozone by as much as 20 ppbv in summer in northern Africa and the Near East. European emissions cause 50–150 additional violations per year (i.e. above those that would occur without European pollution) of the European health standard for ozone (8-h average ...

OBJECTIVE To estimate the prevalence and incidence of dementia in Northern Territory Indigenous and non-Indigenous populations. DESIGN, SETTING AND PARTICIPANTS Four data sources were used to identify clients with a diagnosis of dementia, from 1 January 2008 to 31 December 2011. The data sources included hospital admissions, aged care services, primary care and death registration. A capture-r...

An inverted repeat corresponding to parts of the centrin gene of Chlamydomonas reinhardtii was placed downstream of the NIT1 promoter, which is induced by ammonium starvation. After induction, transformants developed centrin deficiency as assayed by immunofluorescence, Western blotting, and Northern blotting. The effect was reversible, demonstrating that the NIT1 promoter allowed controlled RNA...

A cDNA encoding rat homologue of the previously characterized mouse Sox6 was isolated by a polymerase chain reaction (PCR) cloning strategy. Comparison of this eDNA with homologous mouse, human and rainbow trout cDNA exhibited an overall amino acid sequence identity of 99.6, 89.3 and 76.3% respectively. The leucine-zipper and HMG-box motif were almost completely conserved between these homologu...

Small RNAs (sRNA) of 15-30 nucleotides regulate various processes in eukaryotes. Regulatory sRNAs are produced through the RNA interference (RNAi) pathway from double stranded RNA (dsRNA). Dicer or dicer-like enzyme processes the dsRNAs into 15-30 bp dsRNAs, which in turn bind to the RNA-induced silencing complex (RISC) to regulate gene expression. We previously cloned sRNAs from barley. In thi...