Aim of present study is to evaluate the effect of salinity on the growth of Rhizobium symbiosis with various cultivars of Berseem (WARDAN, BB3, JHB 052 and BB2). Two sets of plots were maintained in which one set contained non-inoculated while another set contained inoculated seeds with Rhizobium culture, which were irrigated with saline waters of different concentrations (0, 3, 6, 7.2, 10, 12 ...

The isoflavones, daidzein and genistein, have been isolated and identified as the major inducers of nod genes of Bradyrhizobium japonicum. The common nod genes of rhizobia are in turn responsible for stimulating root hair curling and cortical root cell division, the earliest steps in the host response. This study evaluated whether there was a relationship between root isoflavonoid production an...

Nodulation ability of 15 Rhizobium strains isolated from root and stem nodules of tropical Aeschynomene species was studied on 20 different Aeschynomene species and four other legumes-Arachis hypogaea, Stylosanthes guianensis, Macroptilium atropurpureum, and Sesbania rostrata. The results of this investigation showed that Aeschynomene species could be divided into three groups according to the ...

Isolation and Nodulation Rhizobium sp. from Pueraria javanica (Benth.) Benth. Study of Its Survival on Peat Soil Compost Oil Palm Empty Fruit Bunch was done March to July 2008, in the Laboratory Plant Biotechnology Indonesian Research Institute (IOPRI) Marihat Station Pematang Siantar, Microbiology, Department Biology, FMIPA USU. The sampel nodules were taken roots growing oil palm plantation a...

The presence of nitrogen inhibits legume nodule formation, but the mechanism of this inhibition is poorly understood. We found that 2.5 mM nitrate and above significantly inhibited nodule initiation but not root hair curling in Medicago trunatula. We analyzed protein abundance in M. truncatula roots after treatment with either 0 or 2.5 mM nitrate in the presence or absence of its symbiont Sinor...

Common bean (Phaseolus vulgaris L.) genotype Flamingo was inoculated with Rhizobium tropici CIAT899 and Glomus intraradices by mycorrhizal inoculant or by contact with mycorrhized Stylosanthes guianensis and grown under sufficient versus deficient phosphorus supply after transfer from initial sand culture, for comparing the effects of double inoculation (by contact or by inoculant) on growth, n...

Establishment of the nitrogen-fixing nodulation symbiosis between legumes and rhizobia requires plant-wide reprogramming to allow infection and development of nodules. Nodulation is regulated principally via a mechanism called autoregulation of nodulation (AON). AON is dependent on shoot and root factors and is maintained by the nodulation autoregulation receptor kinase (NARK) in soybean. We de...

Cytokinins are essential for legume plants to establish a nitrogen-fixing symbiosis with rhizobia. Recently, the expression level of cytokinin biosynthesis IPTs (ISOPENTENYLTRANSFERASES) genes was shown to be increased in response to rhizobial inoculation in Lotus japonicus, Medicago truncatula and Pisum sativum. In addition to its well-established positive role in nodule primordium initiation ...

We isolated a recessive symbiotic mutant of Lotus japonicus that defines a genetic locus, LOT1 (for low nodulation and trichome distortion). The nodule number per plant of the mutant was about one-fifth of that of the wild type. The lot1 mutant showed a moderate dwarf phenotype and distorted trichomes, but its root hairs showed no apparent differences to those of the wild type. Infection thread...

The study evaluated the response of alfalfa (Medicago sativa L) to arbuscular mycorrhizal fungi (AM) species Glomus intraradices and Sinorhizobium meliloti, strain 1021 regarding the dry biomass accumulation, mycorrhizal fungi colonization, nodulation and nitrogen fixation activity. Alfalfa plants were grown in a glasshouse until the flowering stage (58 days), in 4 kg plastic pots using leached...