These investigations originated in studying the basis of a method for microbiological assay of nicotinic acid (McIlwain & Stanley, 1948). The method was that in which acid produced from glucose by Lactobacillus arabinosus 17-5 is titrated with alkali after a period of incubation of about 3 days. The assay depends on the empirical observation that the quantity of titratable acid produced during ...

Barbara Upmeier, Jürgen E. Thomzik*, and Wolfgang Barz Lehrstuhl für Biochemie der Pflanzen, Westfälische Wilhelms-Universität Münster, Hindenburgplatz 55, D-4400 Münster, Bundesrepublik Deutschland Z. Naturforsch. 43c, 835 -842 (1988); received August 1/September 30, 1988 Petroselinum hortense, Umbelliferae, Plant Cell Cultures, Nicotinic Acid, Nicotinic Acid-Nglucoside A soluble enzyme cataly...

The pyridine moieties of the pyridine nucleotides, nicotinic acid mononucleotide, nicotinic acid adenine dinucleotide, and nicotinamide adenine dinucleotide, were shown to be incorporated into ricinine by Ricinus communis L. with an efficiency comparable to that of quinolinic acid, nicotinic acid, and nicotinamide, which had been established previously as being efficient precursors of this alka...

Nicotinic acid (niacin) has long been used as an antidyslipidemic drug. Its special profile of actions, especially the rise in HDL-cholesterol levels induced by nicotinic acid, is unique among the currently available pharmacological tools to treat lipid disorders. Recently, a G-protein-coupled receptor, termed GPR109A (HM74A in humans, PUMA-G in mice), was described and shown to mediate the nic...

The inability of vitamin Be-deficient rats and mice to convert dietary tryptophan to nicotinic acid and its metabolites has been observed by several workers (l-4). Further, when vitamin Be supplements were given to rats previously deficient in the vitamin, the animals usually recovered the ability to transform tryptophan into nicotinic acid metabolites, although the response was apparently infl...

This study reports on the functional expression of a specific, high-affinity carrier-mediated mechanism for the transport of niacin (nicotinic acid) in human liver cells. Both human-derived liver HepG2 cells and human primary hepatocytes were used as models in these investigations. The initial rate of transport of nicotinic acid into HepG2 cells was found to be acidic pH, temperature, and energ...

Yeasts utilize tryptophan for the synthesis of nicotinic acid under aerobic, but not under anaerobic, conditions. Under aerobic conditions of growth, radioactivity from tryptophanJ4C uniformly labeled in the benzene ring and 14C!-carboxyl-labeled 3-hydroxyanthranilic acid is incorporated into nicotinic acid. Under anaerobic conditions, little radioactivity from these compounds is incorporated i...

The pyridine moieties of the pyridine nucleotides, nicotinic acid mononucleotide, nicotinic acid adenine dinucleotide, and nicotinamide adenine dinucleotide, were shown to be incorporated into ricinine by Ricinus communis L. with an efficiency comparable to that of quinolinic acid, nicotinic acid, and nicotinamide, which had been established previously as being efficient precursors of this alka...

The addition of corn products to a synthetic low protein diet very low in nicotinic acid has been reported by Krehl and coworkers (1,2) to depress growth in rats. This growth retardation could be prevented by tryptophan as well as by nicotinic acid. These findings have been confirmed in this laboratory by the paired feeding technique (3) and by other investigators (4, 5). The interchangeability...