The CLE family (CLAVATA3/embryo surrounding region-related), a class of small secreted proteins, play important roles in plant development and stress responses. Members the have been characterized number species, including Arabidopsis rice. However, limited information is available about peptides tobacco (Nicotiana tabacum) related Nicotiana species. Here we report identification 84 members thr...

Isolated, nick-translated Pvu II fragments of Nicotiana tabacum chloroplast DNA produce specific intra- and intergeneric hybridization signals with chloroplast DNA digests from several representatives of the Solanaceae. These data, along with similarities in restriction enzyme patterns, permit construction of physical maps for Nicotiana line 92 (a cytoplasmic substitution line), Atropa belladon...

We report cell-to-cell movement of mitochondria through a graft junction. Mitochondrial movement was discovered in an experiment designed to select for chloroplast transfer from Nicotiana sylvestris into Nicotiana tabacum cells. The alloplasmic N. tabacum line we used carries Nicotiana undulata cytoplasmic genomes, and its flowers are male sterile due to the foreign mitochondrial genome. Thus, ...

Origin and rearrangement of ribosomal DNA repeats in natural allotetraploid Nicotiana tabacum are described. Comparative sequence analysis of the intergenic spacer (IGS) regions of Nicotiana tomentosiformis (the paternal diploid progenitor) and Nicotiana sylvestris (the maternal diploid progenitor) showed species-specific molecular features. These markers allowed us to trace the molecular evolu...

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RNA editing in plant organelles is mediated by site-specific, nuclear-encoded factors. Previous data suggested that the maintenance of these factors depends on the presence of their rapidly evolving cognate sites. The surprising ability of allotetraploid Nicotiana tabacum (tobacco) to edit a foreign site in the chloroplast ndhA messenger RNA was thought to be inherited from its diploid male anc...

Gesellschaft für Biotechnologische Forschung, Mascheroder Weg, D-3300 Braunschweig-Stöckheim, Bundesrepublik Deutschland Z. Naturforsch. 42c, 69—72 (1987); received August 21/September 22, 1986 Agrobacterium rhizogenes, Alkaloids, Nicotine, Cytisine, Nicotiana, Spartium The growth of root hairs from stems of Nicotiana tabacum and Spartium junceum was induced by Agrobacterium rhizogenes. Stem ro...