newcastle disease virus (ndv) is known as one of the most important endemic viral pathogen for various avian species such as ostrich, in iran. therefore, establishing a routine vaccination program against nd in ostrich flocks would be useful in order to reduce the danger of this infection. newcastle disease occurs among the ostriches and leads to high rate of mortality while most of the losses ...

background and aims: newcastle disease virus (ndv) infection have been established in at least 241species of birds representing 27 of the 50 orders of the class.ndv isolate were obtained from infected ostrich flock during the outbreaks of nd in iran 2012. the f gene fragment which codes the main functional region of the f protein was obtained by rt-pcr and sequenced. methods: from the pathotype...

Recent advances in reverse genetics techniques make it possible to manipulate the genome of RNA viruses such as Newcastle disease virus (NDV). Several NDV vaccine strains have been used as vaccine vectors in poultry, mammals, and humans to express antigens of different pathogens. The safety, immunogenicity, and protective efficacy of these NDV-vectored vaccines have been evaluated in pre-clinic...

Background and Aims: Newcastle disease virus (NDV) infection have been established in at least 241species of birds representing 27 of the 50 orders of the class.NDV isolate were obtained from infected ostrich flock during the outbreaks of ND in Iran 2012. The F gene fragment which codes the main functional region of the F protein was obtained by RT-PCR and sequenced. Methods: From the pathotype...

The marked progress in recombinant deoxyribonucleic acid (DNA) technology during the past decade has led to the development of a variety of safe new vaccine vectors which are capable of efficiently expressing foreign immunogens. These have been based on a variety of virus types--poxviruses, herpesviruses and adenoviruses--and have led to the production of many new potential recombinant vaccines...

Newcastle disease (ND) is one of the most serious illnesses of chickens. Live vaccines are widely used to prevent chicken from the disease all over the world. To access the effective and potentiate ND vaccine, a homogenous subpopulation from LaSota strain was selected following cultivation of the virus on primary chicken embryofibroblast (CEF) cells. Pathogenicity indices and molecular characte...

Since 2002, following its introduction, the lineage 5d Newcastle disease virus (so-called Goose paramyxovirus - GPMV) strain has caused numerous disease outbreaks among commercial and backyard poultry in South Africa, raising questions about the ability of commercially available Newcastle disease vaccines to fully protect poultry against the strain. This study aimed to determine whether there a...

in order to adapt lasota strain of newcastle disease virus (ndv) on chick embryo fibroblast (cef) cells, 0.1 ml from lasota vaccine produced in razi vaccine and serum research institute inoculated to the cef cells and was passaged five times in the cef grown in eagle’s minimum essential medium (mem). first to third passages were blind but in fourth passage cytopathic effect of virus was observe...

newcastle disease (nd) is one of the most serious illnesses of chickens. live vaccines are widely used to prevent chicken from the disease all over the world. to access the effective and potentiate nd vaccine, a homogenous subpopulation from lasota strain was selected following cultivation of the virus on primary chicken embryofibroblast (cef) cells. pathogenicity indices and molecular characte...