نتایج جستجو برای: ndna

تعداد نتایج: 329  

Journal: :Acta medica Okayama 1977
H Ogura T Oda

The intracellular localization of the avian myeloblastosis virus (AMV) genome was studied. Nuclear and mitochondrial DNAs from myeloblasts were examined by hybridization with 32P labeled AMV-RNA of high molecular weight for the presence of virus specific DNA sequences. Nuclear DNA (nDNA) from myeloblasts specifically hybridized with viral RNA, whereas purified closed circular mitochondrial DNA ...

Journal: : 2023

DNA isolation from blood is a commonly used application to obtain nDNA and mtDNA. It was previously shown that could be performed the pellet obtained after centrifugation of freeze-thawed (FTB), this pretreatment had constructive results on isolation. However, which levels can for pretreatment, their effects are unknown. The aim study determine appropriate show isolated DNA. For purpose, isolat...

Journal: :iranian journal of child neurology 0
mahmoud reza ashrafi 1. pediatric neurology department, children’s medical center, pediatric center of excellence, tehran university of medical science, tehran, iran alireza tavasoli 1. pediatric neurology department, children’s medical center, pediatric center of excellence, tehran university of medical science, tehran, iran

pls see pdf.

2015
Deborah M. Money Emily C. Wagner Evelyn J. Maan Tessa Chaworth-Musters Izabelle Gadawski Julie E. van Schalkwyk John C. Forbes David R. Burdge Arianne Y. K. Albert Zoe Lohn Hélène C. F. Côté Dhayendre Moodley

INTRODUCTION Combination antiretroviral therapy (cART) can effectively prevent vertical transmission of HIV but there is potential risk of adverse maternal, foetal or infant effects. Specifically, the effect of cART use during pregnancy on mitochondrial DNA (mtDNA) content in HIV-positive (HIV+) women is unclear. We sought to characterize subclinical alterations in peripheral blood mtDNA levels...

Journal: :Critical Care 2007
Hélène CF Côté Andrew G Day Daren K Heyland

BACKGROUND Mitochondrial dysfunction may be causally related to the pathogenesis of organ failure in critically ill patients. Decreased mitochondrial DNA (mtDNA) levels have been associated with mitochondrial dysfunction and were investigated here in relation to short-term (31-day) survival. METHODS This was a prospective longitudinal cohort study of 28 mechanically ventilated critically ill ...

Journal: :FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2000
G Barja A Herrero

DNA damage is considered of paramount importance in aging. Among causes of this damage, free radical attack, particularly from mitochondrial origin, is receiving special attention. If oxidative damage to DNA is involved in aging, long-lived animals (which age slowly) should show lower levels of markers of this kind of damage than short-lived ones. However, this possibility has not heretofore be...

2014
Liron Levin Amit Blumberg Gilad Barshad Dan Mishmar

Most cell functions are carried out by interacting factors, thus underlying the functional importance of genetic interactions between genes, termed epistasis. Epistasis could be under strong selective pressures especially in conditions where the mutation rate of one of the interacting partners notably differs from the other. Accordingly, the order of magnitude higher mitochondrial DNA (mtDNA) m...

2015
Malgorzata Karbownik-Lewinska Jan Stepniak Magdalena Milczarek Andrzej Lewinski

PURPOSE Iodine, bivalent iron (Fe²⁺), and hydrogen peroxide (H₂O₂), all significantly affecting the red-ox balance, are required for thyroid hormone synthesis. Intracellular iodine excess (≥10⁻³ M) transiently blocks thyroid hormonogenesis (an adaptive mechanism called Wolff-Chaikoff effect). The aim of the study was to evaluate the effects of iodine, used as potassium iodide (KI) or potassium ...

2013
Laurent Chatre Miria Ricchetti

Nuclear (nDNA) and mitochondrial DNA (mtDNA) communication is essential for cell function, but it remains unclear whether the replication of these genomes is linked. We inspected human cells with a novel fluorescence in situ hybridization protocol (mitochondrial Transcription and Replication Imaging Protocol) that identifies mitochondrial structures engaged in initiation of mtDNA replication an...

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